高氯酸盐对高脂膳食小鼠脂质代谢的影响

Effects of perchlorate on lipid metabolism in high-fat diet mice

目的探究高氯酸盐对高脂膳食小鼠脂质代谢的影响。方法将C57BL/6J小鼠分为4组,分别为对照组(CG)、高氯酸盐低剂量组[LG,每天0.1 mg/(kg·bw)]、中剂量组[MG,每天1.0 mg/(kg·bw)]和高剂量组[HG,每天10.0 mg/(kg·bw)],连续暴露12周,通过对肝脏油红O染色、血生化指标检测、脂代谢调控酶测定和脂肪酸组成及含量测定进行高氯酸盐对高脂膳食小鼠脂质代谢的评估。结果高于1.0mg/(kg·bw)的暴露剂量可在小鼠肝脏内形成脂滴累积,总胆固醇、甘油三酯和高密度脂蛋白胆固醇含量均显著性升高(P<0.05),并且HG组脂质代谢关键调控酶乙酰辅酶A羧化酶(acetyl-CoAcarboxylase,ACC)和脂肪酸合成酶(fattyacidsynthase,FAS)含量显著性升高(P<0.05),表明高氯酸盐可能会促进脂肪的合成。随着高氯酸盐暴露浓度的增加,棕榈酸(C16:0)、珍珠酸(C17:0)、硬脂酸(C18:0)、花生四烯酸(C20:4)和二十二碳六烯酸(C22:6)体内水平显著下降,而棕榈亚酸(C16:1)、油酸(C18:1)和亚油酸(C18:2)体内水平显著上升,饱和脂肪酸显著下降,不饱和脂肪酸显著上升。结论高氯酸盐可能会造成小鼠肝脏损伤和脂代谢紊乱,且随着暴露剂量的升高肝脏损伤越显著。本研究为高氯酸盐体内毒性损伤和健康效应的研究提供了理论基础。

Objective To explore the effect of perchlorate on lipid metabolism in mice fed with high-fat diet.Methods C57BL/6J mice were divided into 4 groups:Control group(CG),perchlorate low-dose group[LG,0.1 mg/(kg·bw)],medium-dose group[MG,1.0 mg/(kg·bw)]and high-dose group[HG,10.0 mg/(kg·bw)].After 12 weeks intervention,assessment of perchlorate on lipid metabolism in mice fed a high-fat diet was assessed by liver oil red O staining,blood biochemical index assays,lipid metabolism regulatory enzyme assays and fatty acid composition and content measurements.Results Doses of perchlorate exposure above 1.0 mg/(kg·bw)resulted in lipid droplet accumulation in the liver of mice,with significant increases in total cholesterol,triglycerides and high-density lipoprotein cholesterol levels(P<0.05),and significant increases in acetyl-CoA carboxylase(ACC)and fatty acid synthase(FAS),key regulatory enzymes of lipid metabolism,in the HG group(P<0.05).These results indicated that perchlorate might promote fat synthesis.With increasing concentrations of perchlorate exposure,the levels of palmitic acid(C16:0),pearlic acid(C17:0),stearic acid(C18:0),arachidonic acid(C20:4)and docose hexaenoie acid(C22:6)decreased significantly,while the levels of palmitic acid(C16:1),oleic acid(C18:1)and linoleic acid(C18:2)increased significantly,and saturated fatty acids decreased significantly and a significant increase in unsaturated fatty acids.Conclusion Perchlorate may cause liver damage and disturbance of lipid metabolism in C57BL/6J mice fed a high-fat diet,and the liver damage become more pronounced with increasing exposure dose.This article provide a theoretical basis for the study of perchlorate toxicity and health effects in vivo.