子痫前期中性粒细胞胞外诱捕网的形成与血管内皮损伤的相关性研究

Correlation between the formation of the extracellular trap network of preeclamptic neutrophils and vascular endothelial injury

目的 研究子痫前期(PE)病人血清中中性粒细胞胞外诱捕网(NETs)来源,并分析与血管内皮损伤的相关性。方法选取2020年9月至2021年9月潍坊市人民医院收治的30例PE病人作为PE组,30例正常分娩的健康产妇作为Nor-P组,同期于该院进行孕前检查的健康未孕妇女30例作为对照组。比较三组的血白细胞及中性粒细胞数量、髓过氧化物酶、NETs、内皮损伤指标含量的差异。采用Pearson检验评估NETs与内皮损伤的相关性。结果 PE组白细胞数量(12.01±0.88)×10^(9)/L、中性粒细胞数量(10.22±0.99)×10^(9)/L及髓过氧化物酶(15.39±0.84)μg/L、NETs(124.68±3.63)ng/L、血管性血友病因子(vWF)(122.79±4.05)μg/L水平高于对照组和Nor-P组(P<0.05)。Pearson相关性分析发现,PE病人血清NETs水平与中性粒细胞数量呈正相关(r=0.63,P<0.05),同时NETs水平与vWF水平呈正相关(r=0.83,P<0.05)。结论 PE病人血清中的NETs水平异常升高,与中性粒细胞数量升高有关,具体水平可反应血管内皮损伤程度。

Objective To investigate the source of neutrophil extracellular trap networks(NETs)in the serum of patients with preeclampsia(PE)and to analyze their correlation with vascular endothelial injury.Methods A total of 30 patients with PE admitted to Weifang People's Hospital from September 2020 to September 2021 were selected as the PE group,30 healthy women with normal delivery were selected as the Nor-P group,and 30 healthy nonpregnant women who underwent prepregnancy examination at the hospital during the same time were selected as the control group.The differences in blood leukocyte and neutrophil counts,myeloperoxidase,NETs and endothelial injury index levels were compared among the three groups.The Pearson test was used to assess the correlation between NETs and endothelial injury.Results The levels of leukocyte count(12.01±0.88)×10^(9)/L,neutrophil count(10.22±0.99)×10^(9)/L,and myeloperoxidase(15.39±0.84)μg/L,NETs(124.68±3.63)ng/L and von Willebrand factor(vWF)(122.79±4.05)μg/L in the PE group were higher than those in the control and Nor-P groups(P<0.05).Pearson correlation analysis showed that there was a positive correlation between serum NET levels and neutrophil count in PE patients(r=0.63,P<0.05),as well as a positive correlation between NET levels and vWF levels(r=0.83,P<0.05).Conclusion Abnormally elevated levels of NETs in the serum of PE patients are associated with elevated neutrophil counts,and the specific levels may reflect the degree of vascular endothelial injury.