CUL4相关因子7、微RNA-589-5p在结直肠癌组织中表达与临床病理特征及预后的关系

The relationship between the expression of DCAF7 and miR-589-5p in colorectal cancer and clinicopathological characteristics and prognosis

目的 分析结直肠癌组织中CUL4相关因子7(DCAF7)、微RNA-589-5p(miR-589-5p)表达水平,并探讨其与结直肠癌临床病理特征及预后的关系。方法 纳入2018年9月至2019年9月于宝鸡市人民医院行结直肠癌根治术的78例。采用实时荧光定量PCR法检测组织中miR-589-5p、DCAF7 mRNA表达水平。免疫组化法检测组织中DCAF7表达。Spearman法分析组织miR-589-5p与DCAF7的相关性。分析miR-589-5p、DCAF7水平与结直肠癌临床病理特征的关系。Kaplan-Meier法分析结直肠癌组织miR-589-5p、DCAF7表达与病人预后的关系,多因素Cox回归分析影响结直肠癌预后的因素。结果 Ualcan数据库中,结肠腺癌组织中miR-589表达水平低于癌旁组织(P<0.05),DCAF7表达水平高于癌旁组织(P<0.05)。与癌旁组织相比,结直肠癌组织中DCAF7阳性表达率及DCAF7 mRNA相对表达水平(2.79±0.84比1.02±0.31)较高(P<0.05),miR-589-5p相对表达水平(0.44±0.09比1.04±0.22)较低(P<0.05)。Spearman相关分析显示,组织中miR-589-5p与DCAF7表达水平呈负相关(P<0.05)。miR-589-5p、DCAF7均与淋巴结转移、肿瘤浸润深度相关(P<0.05)。Kaplan-Meier法显示,miR-589-5p高表达组病人平均生存时间显著长于miR-589-5p低表达结直肠癌病人(P<0.05),DCAF7阴性表达病人平均生存时间显著长于DCAF7阳性表达病人(P<0.05)。多因素分析表明,淋巴结有转移、miR-589-5p低表达、DCAF7阳性是影响结直肠癌病人不良预后的独立危险因素(P<0.05)。与Control组比,miR mimic组miR-589-5p表达(2.58±0.32比1.02±0.12)显著升高(P<0.05),DCAF7表达(0.37±0.06比1.01±0.12)、侵袭细胞数(60.88±9.94比178.52±26.08)个显著降低(P<0.05);与miR mimic组比,miR mimic+pc-DCAF7组DCAF7表达、侵袭细胞数显著升高(P<0.05)。结论 结直肠癌组织中DCAF7呈高表达、miR-589-5p呈低表达,与结直肠癌淋巴结转移、肿瘤浸润深度以及不良预后有关。

Objective To analyze the expression levels of DCAF7 and microRNA-589-5p(miR-589-5p)in colorectal cancer tissues,and to explore their relationship with the clinicopathological characteristics and prognosis of colorectal cancer.Methods Seventyeight patients who underwent radical resection of colorectal cancer in Baoji People's Hospital from September 2018 to September 2019 were enrolled.Real-time fluorescence quantitative PCR was used to detect the expression levels of miR-589-5p and DCAF7 mRNA in the tissues.Immunohistochemical method was used to detect the expression of DCAF7 in the tissues.Spearman method was used to analyze the correlation between tissue miR-589-5p and DCAF7.The relationship between miR-589-5p,DCAF7 levels and clinicopathological characteristics of colorectal cancer was analyzed.Kaplan-Meier method was used to analyze the relationship between the expression of miR-589-5p and DCAF7 in colorectal cancer tissues and the prognosis of patients,and multivariate Cox regression was used to analyze the factors affecting the prognosis of colorectal cancer.Results In the Ualcan database,the expression level of miR-589 in colon adenocarcinoma tissue was lower than that in adjacent tissues(P<0.05),and the expression level of DCAF7 was higher than that in adjacent tissues(P<0.05).Compared with adjacent tissues,the positive expression rate of DCAF7 and the relative expression level of DCAF7 mRNA(2.79±0.84 vs.1.02±0.31)in colorectal cancer tissue were higher(P<0.05),and the relative expression level of miR-589-5p(0.44±0.09 vs.1.04±0.22)was lower(P<0.05).Spearman correlation analysis showed that the expression levels of miR-589-5p and DCAF7 in tissues were negatively correlated(P<0.05).Both miR-589-5p and DCAF7 were related to lymph node metastasis and the depth of tumor invasion(P<0.05).Kaplan-Meier method showed that the average survival time of patients in the miR-589-5p high expression group was significantly longer than that in colorectal cancer patients with miR-589-5p low expression(P<0.05),the average survival time of patients with negative expression of DCAF7 was significantly longer than that of patients with positive expression of DCAF7(P<0.05).Multivariate analysis showed that lymph node metastasis,low expression of miR-589-5p,and positive DCAF7 were independent risk factors affecting the poor prognosis of patients with colorectal cancer(P<0.05).Compared with the control group,the expression of miR-589-5p(2.58±0.32 vs.1.02±0.12)in the miR mimic group was significantly increased(P<0.05),the expression of DCAF7(0.37±0.06 vs.1.01±0.12)and the number of invasive cells(60.88±9.94 vs.178.52±26.08)individual were significantly decreased(P<0.05);Compared with the miR mimic group,the expression of DCAF7 and the number of invasive cells were significantly increased(P<0.05).Conclusion DCAF7 is highly expressed in colorectal cancer tissues,and miR-589-5p is lowly expressed.They are related to lymph node metastasis,depth of tumor invasion and poor prognosis in colorectal cancer.