Toll样受体5基因rs2072493与肝癌易感性的关系

Relationship between SNP rs2072493 in TLR5 and susceptibility to primary liver cancer

目的:探讨Toll样受体5基因单核苷酸多态性(single nucleotide polymorphism SNP)rs2072493位点与肝癌遗传易感性的关系。方法:采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)法检测205例肝癌病例组和238例正常对照组Toll样受体5基因SNP rs2072493的基因型分布。用非条件性Logistic回归计算比值比(OR)及其95%置信区间评估在共显性、显性、隐性、超显性四种遗传模式下rs2072493与肝癌发病风险的关系。结果:在共显性、显性、隐性遗传模式下,Toll样受体5基因SNP rs2072493与肝癌的发病风险有关联。其中共显性遗传模式最适合,AIC和BIC最小。与携带AA基因型者相比,携带GA和GG基因型者肝癌的发病风险降低(GA vs AA:OR=0.512,95%CI=0.333-0.787、GG vs AA:OR=0.192,95%CI=0.077-0.477)。结论:Toll样受体5基因SNP rs2072493与肝癌发病风险关联。

Objective:To investigate the relationship between SNP rs2072493 in Toll-like receptor 5(TLR5)gene and genetic susceptibility to primary liver cancer.Methods:Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)method was used to detect the genotype distributions of SNP rs2072493 in 205 patients with primary liver cancer and 238 normal people.The odds ratio(OR)and 95%confidence interval(CI)were calculated by non-conditional logistic regression to evaluate the relationship between rs2072493 and the risk of primary liver cancer under the four genetic models of Codominant,Dominant,Recessive and Overdominant.Results:SNP rs2072493 was associated with the risk of primary liver cancer under Codominant,Dominant and Recessive genetic models.The codominant genetic model was the best model with the lowest AIC and BIC value.Compared with AA genotype,GA and GG genotype could reduce the risk of primary liver cancer(GA vs AA:OR=0.512,95%CI=0.333-0.787;GG vs AA:OR=0.192,95%CI=0.077-0.477).Conclusion:SNP rs2072493 in TLR5 is associated with the risk of primary liver cancer.