基于TCGA数据库分析DDX27在肝细胞癌中的预后价值

Analysis of the prognostic value of DDX27 in hepatocellular carcinoma based on TCGA database

目的 基于癌症基因图谱(TCGA)数据库,分析DEAD-BOX RNA解旋酶(DDX27)基因在肝细胞癌中的表达、临床意义以及分子信号通路。方法 通过TCGA数据库下载的肝细胞癌相关的表达数据和临床特征数据,运用R.4.0.3软件和Strawberry Perl 5.32.0.1提取肝癌组织和正常组织中DDX27的表达量数据,使用Mann-Whitney U检验比较两组间的表达差异。以DDX27表达量中位值为界将临床资料分为高表达组和低表达组,采用卡方检验分析表达量与临床特征之间的关系。分别进行单因素和多因素cox回归分析,使用Kaplan-Meier法进行生存分析并采用多种在线数据库验证,利用基因富集分析(GSEA)预测DDX27在肝细胞癌中参与的信号通路。结果 肝细胞癌组织中的DDX27表达量显著高于正常组织,差异有统计学意义(P<0.05),单因素cox回归分析发现DDX27表达与Stage分期(P<0.05)和T分期(P<0.05)有关。多因素cox回归分析得到DDX27(HR=1.07,95%CI:1.02~1.13,P<0.05)可以作为肝细胞癌的独立预后因素。DDX27高表达组的总生存率显著低于低表达组,差异有统计学意义(P<0.05),与多个在线数据库验证结果一致。GSEA结果表明,高表达的DDX27主要参与RNA降解、细胞周期、嘌呤代谢、同源重组、核苷酸切除修复及胞质DNA传感通路等与癌症发生的分子信号通路。结论 DDX27在肝细胞癌患者中高表达,与肝细胞癌的预后相关,可以作为肝细胞癌预后的新型分子标志物。

Objective To analyze the expression of DEAD-BOX RNA helicase(DDX27)in hepatocellular carcinoma patients and normal tissues based on The Cancer Genome Atlas(TCGA)database,to investigate the correlation between DDX27 and clinical characteristics,prognosis and to predict signal pathway of hepatocellular carcinoma.Methods The expression data and clinical characteristics of hepatocellular carcinoma were downloaded from TCGA database.The expression data of DDX27 in hepatocellular carcinoma tissues and normal tissues were extracted by R.4.0.3 software and Strawberry Perl 5.32.0.1,and the expression difference between the two groups were compared by Mann-Whitney U test.DDX27 expression was divided into the high expression group and the low expression group according to the median.Chi-square test was used to analyze the relationship between expression and clinical characteristics.Univariate and multivariate cox regression analysis were used to evaluate the prognostic significance.Kaplan-Meier was used for survival analysis and multiple online databases to verify.Gene enrichment analysis(GSEA)was used to predict the signal pathway of DDX27 in hepatocellular carcinoma.Results The expression of DDX27 in the hepatocellular carcinoma group was significantly higher than that in the normal control group(P<0.05).The expression of DDX27 in hepatocellular carcinoma patients was related to Stage and T stage.DDX27(HR=1.07,95% CI:1.02~1.13,P<0.05)can be used as an independent prognostic factor for hepatocellular carcinoma.The overall survival rate of the group with high expression of DDX27 was lower than that of the group with low expression of DDX27(P<0.05),which was consistent with the results of online database.GSEA enrichment analysis showed highly expressed DDX27 was mainly involved in RNA degradation,cell cycle,purine metabolism,homologous recombination,nucleotide excision repair and cytoplasmic DNA sensing pathways and other molecular signaling pathways related to cancer.Conclusion DDX27 is highly expressed in patients with hepatocellular carcinoma,which is related to the prognosis of hepatocellular carcinoma,and can be used as a new molecular marker for the prognosis of hepatocellular carcinoma.