外周血NLR、VEGF、miRNA let-7a水平与喉癌患者淋巴结转移的相关性

Correlation between expression levels of peripheral blood NLR,VEGF and miRNA let-7a and lymph node metastasis in patients with laryngeal cancer

目的 分析外周血中性粒细胞/淋巴细胞比例(NLR)、血管内皮生长因子(VEGF)、miRNA let-7a表达水平与喉癌患者淋巴结转移的相关性。方法 选取2018年1月至2021年1月运城第一医院收治的60例喉癌患者作为喉癌组,30例喉部良性病变患者为对照组,另选取同期来院体检的健康人30名为健康组,比较三组外周血NLR、VEGF、miRNA let-7a。根据淋巴结转移情况将喉癌患者分为有淋巴结转移亚组和无淋巴结转移亚组,比较两个亚组之间外周血NLR、VEGF、miRNA let-7a,绘制受试者工作特征曲线(ROC)曲线评估NLR、VEGF、miRNA let-7a水平对喉癌患者有无淋巴结转移的诊断价值。结果 外周血NLR、VEGF水平:喉癌组>对照组>健康组,miRNA let-7a:喉癌组<对照组<健康组,差异均有统计学意义(F=110.518、727.392、402.508,P<0.05)。60例喉癌患者中无淋巴结转移28例,淋巴结转移32例。有淋巴结转移患者NLR、VEGF水平均明显高于淋巴结转移患者,miRNA let-7a表达明显低于无淋巴结转移患者,差异有统计学意义(t=5.078、9.956、6.353,P<0.05)。ROC结果显示,NLR、VEGF、miRNA let-7a及其联合检测的AUC分别为0.733、0.807、0.752、0.894。结论 喉癌患者外周血NLR、VEGF、miRNA let-7a表达异常,且均与淋巴结转移有关,联合检测NLR、VEGF及miRNA let-7a有助于提高喉癌淋巴结转移诊断效能。

Objective To analyze the correlation between expression levels of peripheral blood neutrophil/lymphocyte ratio(NLR),vascular endothelial growth factor(VEGF)and miRNA let-7a and lymph node metastasis in patients with laryngeal cancer.Methods 60 patients with laryngeal cancer admitted to Yuncheng First Hospital between January 2018 and January 2021 were selected as the laryngeal cancer group,and 30 patients with benign laryngeal lesions were included as the control group,and 30 healthy people who came to the hospital for physical examination during the same period were selected as the healthy group.The peripheral blood NLR,VEGF and miRNA let-7a were compared among the three groups,and the patients with laryngeal cancer were divided into the lymph node metastasis subgroup and the non-lymph node metastasis subgroup according to the lymph node metastasis status.The peripheral blood NLR,VEGF and miRNA let-7a were compared between the two subgroups.Receiver operating characteristic(ROC)curve was drawn to evaluate the diagnostic value of levels of NLR,VEGF and miRNA let-7a on presence or absence of lymph node metastasis in patients with laryngeal cancer.Results The levels of NLR and VEGF in peripheral blood:laryngeal cancer group > control group > healthy group,while the miRNA let - 7a:laryngeal cancer group <control group <healthy group (F=110.518,727.392,402.508,P<0.05). Of the 60 patients with laryngealcancer,28 had no lymph node metastasis and 32 had lymph node metastasis. The levels of NLR and VEGF inpatients with lymph node metastasis were significantly higher than those in patients without lymph nodemetastasis,while the expression of miRNA let - 7a was significantly lower than that in patients without lymphnode metastasis(t=5.078,9.956,6.353,P<0.05). The ROC results showed that the AUC values of NLR,VEGF,miRNA let - 7a and the combined detection were 0.733,0.807,0.752 and 0.894 respectively.Conclusion The expression of NLR,VEGF,and miRNA let-7a in peripheral blood of patients with laryngealcancer is abnormal,and they are related to lymph node metastasis. The combined detection of NLR,VEGF,and miRNA let-7a is helpful to improve the diagnostic efficiency of lymph node metastasis in laryngeal cancer.