广西人群miR-30基因rs16827546 C/T和rs10095483 A/C多态性分布特征及其与血脂水平的关系

Distribution characteristics of polymorphisms of miR-30 gene rs16827546 A/C and rs10095483 A/C in Guangxi population and their association with serum lipid level

目的 探讨miR-30基因单核苷酸多态性(SNP)位点rs16827546 C/T和rs10095483 A/C在广西人群中的分布特点,比较其与其他人群的多态性差异,并统计分析不同基因型的常见血脂检测项目水平。方法 SNPscan法对289例研究对象rs16827546 C/T和rs10095483 A/C位点进行分型,用罗氏全自动生化仪检测研究对象的常见血脂水平。结果 在广西人群中rs16827546 C/T位点存在CC、CT两种基因型;rs10095483 A/C位点存在AA、AC两种基因型。广西人群两位点基因型和等位基因频率与HanpMap计划公布的欧洲人(CEU)、约鲁巴人(YRI)、意大利人(TSI)比较,差异有统计学意义(P<0.05);广西人群rs16827546基因型和等位基因频率与与日本人(JPT)比较,差异有统计学意义(P<0.05);广西人群两位点基因型和等位基因频率与北京汉族人(HCB)比较,差异无统计学意义(P>0.05)。广西人群rs16827546 C/T位点两种基因型血脂之间比较,携带CT基因型的TC和LDL-C与携带CC基因型组比较,差异有统计学意义(P<0.05)。结论 广西人群miR-30基因位点rs16827546 C/T和rs10095483 A/C多态性在不同种族、不同地区人群间存在不同程度的差异,rs16827546 C/T位点多态性与TC和LDL-C水平相关。

Objective To explore distribution situation of miR-30 gene single-nucleotide sites rs16827546C/T and rs10095483A/C polymorphisms in Guangxi population and compare its distribution differences with other populations and to analyze level of common blood lipid indexes in genotypes.Methods SNPscan technology was used to test genotypes of rs16827546C/T and rs10095483A/C in 289 Guangxi population and serum lipid levels were measured by Roche automatic biochemical analyzer.Results There were two genotypes of CC and CT at rs16827546 C/T locus in Guangxi population.And AA and AC two genotypes at rs10095483 A/C locus.Compared with the Europeans(CEU),Yoruba(YRI)and Italians(TSI)announced by the HanpMap project,the genotype and allele frequency of the two loci in Guangxi population had statistically significant differences(P<0.05).The genotype and allele frequencies of rs16827546 in Guangxi population were significantly different from those in Japanese(JPT)(P<0.05).There was no statistically significant difference between the genotype and allele frequency of two loci in Guangxi population and Beijing Han population(HCB)(P>0.05).Comparing the blood lipids of the two genotypes at the rs16827546 C/T site in the Guangxi population,there was a statistically significant difference between the TC and LDL-C carrying the CT genotype and the group carrying the CC genotype(P<0.05).Conclusion The miR-30 gene loci rs16827546 C/T and rs10095483 A/C polymorphisms in Guangxi population have different degrees of differences among different ethnic groups and different regions.