不同配比钙镁离子对垃圾渗滤液厌氧处理中微生物的影响

Effects of various ratios of calcium and magnesium ions on microorganisms during anaerobic treatment of landfill leachate

为揭示金属混合物对垃圾渗滤液厌氧生物处理中微生物的影响,选取了垃圾渗滤液中常见的钙离子和镁离子.利用直接均分射线法设计L1、L2和L3三种不同配比的钙镁离子混合物,通过高通量测序技术分析3种不同配比的钙镁离子混合物对微生物的影响,运用R软件绘制并分析环境因子与微生物间的相关性,借助R软件和Gephi可视化分析微生物间相互关系.结果表明:不同配比的钙镁离子混合物对微生物丰度产生不同的影响,而且环境因子与微生物间存在不同的相关性,微生物间存在不同的相互作用关系;然而,钙镁离子配比为L2时相对配比为L1和L3时,钙镁离子混合物对微生物的影响较小,环境因子与微生物间的相关性较弱,微生物间存在一定的负相关性;微生物间一定的竞争关系可能对体系存在积极作用,且体系中丰度较低的微生物也可能与其它微生物间存在较强的交互作用关系.

During the treatment of landfill leachate,the calcium and magnesium ions were selected to evaluate effects of the mixed metal ions on microorganisms.Three ratios of calcium and magnesium ions(L1,L2 and L3)were designed with the direct equipartition ray method.The high-throughput sequencing was used to analyze effects of these ratios on microorganisms.The results show that the microbial abundance varied with the ratio of calcium ion and magnesium ions.And there were different correlations between environmental factors and microorganisms as well as interactions among microorganisms.However,compared to other two rations,the ratio L2 had smaller effect on microorganisms,and the correlations was weaker between environmental factors and microorganisms.Moreover,there were negative correlations among microorganisms.The competition among microorganisms may have positive effects on microbial system,and the microorganisms with low abundance may also have strong interactions with other microorganisms.