一株太空培育螺旋藻诱变株的皮肤修护和保湿研究

Study on skin repairing and skin moisturizing of a Spirulina Platensis mutant induced by space-flight breeding

利用太空育种方法,培育得到新型的钝顶螺旋藻诱变株H11,超声法提取水溶性胞内多糖,使用斑马鱼胚胎尾鳍检测其皮肤修护能力,使用3D表皮模型检测其对天然保湿因子分泌的影响。实验结果表明,野生株和诱变株螺旋藻多糖得率分别为4.88%和20.32%(干重);野生株和诱变株螺旋藻多糖在0.1 mg/mL浓度下,斑马鱼胚胎尾鳍再生生长率分别提高1.44%和13.12%;在1%浓度下3D表皮模型反式尿刊酸的生成分别增加了16.50%和156.43%。结果表明,螺旋藻诱变株多糖含量远远高于野生株,并且表现出优于野生株螺旋藻的皮肤修护和保湿能力。

With the method of space-flight breeding,a new strain of Spirulina Platensis mutant,H11,was cultivated.The water-soluble intracellular polysaccharides were extracted with ultrasonic method.The effects on skin repairing and natural moisturizing factor(NMF)secretion were measured using zebrafish embryo caudal fin and 3D human epidermis model.The results showed that the polysaccharide content of wild type and mutant Spirulina Platensis was 4.88%and 20.32%(dry weight),respectively.After the application of wild type and mutant Spirulina Platensis,the regrowth rate of zebrafish embryo caudal fin was respectively increased by 1.44%and 13.12%at the concentration of 0.1 mg/mL;the trans-urocanic acid production of 3D human epidermis model was respectively increased by 16.50%and 156.43%at the concentration of 1%.The polysaccharide content of mutant Spirulina Platensis was much higher than that of the wild strain,and meanwhile,the mutant Spirulina Platensis also showed better skin repairing and moisturizing ability.