大樱桃矮化砧木吉塞拉6号的组织培养和快速繁殖技术研究

Study on Tissue Culture and Rapid Propagation of Sweet Cherry Dwarf Rootstock‘Gisela 6’

以吉塞拉6号带腋芽茎段为外植体,对大樱桃矮化砧木吉塞拉6号进行了组织培养和快速繁殖技术研究。结果表明,较佳的启动培养基:MS+0.5 mg/L 6-BA+0.2 mg/L NAA,较佳的增殖培养基:MS+1.0 mg/L 6-BA+0.2 mg/L NAA;较佳的生根培养基:1/2 MS+0.5 mg/L IBA+2.0 g/L AC。生根培养第13天进行试管苗炼苗,第20天进行移栽,移栽后的成活率较高,分别为最佳的生根诱导和移栽时间;粉碎苔藓是吉塞拉6号较好的瓶外生根基质。

The stem segment with axillary buds of Gisela 6 was used as explants,and the tissue culture and rapid propagation techniques of Sweet Cherry Dwarf Rootstock Gisela 6 were studied.The results showed that the better initiating medium was MS+0.5 mg/L 6-BA+0.2 mg/L NAA,and the better proliferative medium was MS+1.0 mg/L 6-BA+0.2 mg/L NAA.The better rooting medium was 1/2 MS+0.5 mg/L IBA+2.0 g/L AC.On the 13th day for rooting culture and on the 20th day for transplanting,the high survival rate was achieved after transplanting,which was the optimal time for root induction and transplanting time.Smash moss was better rooting substrate for Gisela 6 outside the bottle.