NME1在皮肤黑色素瘤中的表达及其与临床预后的相关性分析

The Expression of NME1 in Skin Cutaneous Melanoma and Its Correlation with Clinical Prognosis

为探讨非转移性细胞1(NME1)基因在皮肤黑色素瘤(SKCM)中的表达及其与临床预后的相关性,通过GEPIA数据库及UALCAN数据库分析NME1在SKCM组织中的表达;通过GSCA数据库、GEPIA数据库和TIMER数据库分析NME1表达水平与SKCM患者总生存率的相关性;通过cBioPortal数据库分析SKCM患者中NME1基因的突变情况及其与患者总生存率的相关性;通过STRING数据库分析可能与NME1相互作用的蛋白,并利用Metascape数据库对其进行功能富集分析;通过TIMER数据库分析SKCM中NME1的表达水平与免疫细胞浸润水平的相关性。结果显示:NME1在SKCM组织中表达水平明显高于正常组织;NME1的高表达与转移性SKCM患者较低的总生存率显著相关;NME1基因变异与SKCM患者总生存率无明显相关性;NME1及其相互作用蛋白主要参与ARF6运输通路、嘌呤代谢、细胞发育的调节、DNA代谢等生物学过程;转移性SKCM中NME1的高表达与CD4^(+)T细胞、CD8^(+)T细胞、中性粒细胞、巨噬细胞和树突状细胞较低的免疫浸润水平相关。本研究表明,在转移性SKCM患者中,NME1高表达与较低的免疫细胞浸润水平和总生存率相关。NME1有望成为转移性SKCM免疫细胞浸润和预后相关的生物学标志物。

The purpose of this study is to investigate the expression of non-metastatic cell 1(NME1) in skin cutaneous melanoma(SKCM) and its correlation with clinical prognosis. The expression of NME1 in SKCM was analyzed using GEPIA database and UALCAN database. GSCA database, GEPIA database and TIMER database were used to analyze the relationship between the expression of NME1 and the overall survival rate of SKCM patients. The mutation of NME1 in SKCM and its correlation with the overall survival rate of SKCM patients were analyzed through cBioPortal database. The proteins that may interact with NME1 were analyzed by STRING database, and their functions were enriched by Metascape database. The correlation between the expression of NME1 and immune cells infiltration level in SKCM was analyzed using TIMER database. The results showed that the expression of NME1 in SKCM was significantly higher than that in normal tissues. The high expression of NME1 was significantly related to the lower overall survival rate of metastatic SKCM patients. NME1 gene mutation has no significant correlation with the overall survival rate of SKCM patients. NME1 and its interacting proteins are mainly involved in ARF6 trafficking pathway, purine metabolism, regulation of cell development, DNA metabolic process and other biological processes. The high expression of NME1 in metastatic SKCM was significantly correlated with the lower infiltration level of CD4^(+)T cell, CD8^(+)T cell, neutrophil, macrophage and dendritic cell. This study shows that the high expression of NME1 is related to the lower level of immune infiltrating cells, and the overall survival rate in patients with metastatic SKCM. NME1 is expected to become a biomarker related to the infiltration of immune cells and prognosis of metastatic SKCM.