miR-579-3p过表达抑制宫颈癌HeLa细胞增殖、迁移和侵袭的研究

Overexpression of miR-579-3p inhibited the proliferation,migration and invasion of cervical cancer HeLa cells

目的探讨miR-579-3p在宫颈癌HeLa细胞中的表达及对宫颈癌细胞增殖、迁移和侵袭的影响,及其潜在机制。方法将细胞分为H8组[正常培养正常宫颈上皮永生化细胞(H8细胞)]、HeLa组[正常培养宫颈癌细胞(HeLa细胞)]、HeLa+mimic-NC(HeLa细胞转染mimic-NC)和HeLa+mimic-miR-579组(HeLa细胞转染mimic-miR-579-3p)。用细胞计数试剂盒检测各组细胞的增殖情况,用实时荧光定量聚合酶链反应检测miR-579-3p和上皮细胞-间充质转化(EMT)相关基因mRNA的表达水平,用迁移实验和侵袭小室法检测细胞的迁移和侵袭情况。结果H8组中miR-579-3p mRNA相对表达水平(1.00±0.04)显著高于HeLa组(0.12±0.01),差异有统计学意义(P<0.001)。HeLa+mimic-NC组和HeLa+mimic-miR-579组的miR-579-3p mRNA相对表达水平分别为0.13±0.01和0.83±0.10,第2天的增殖能力分别为165.91±9.46和111.35±9.66,侵袭细胞数分别为(124.29±11.22)和(48.29±6.58)个,迁移细胞数分别为(83.86±7.22)和(47.86±6.26)个,Viementin mRNA相对表达水平分别为3.24±0.38和1.49±0.15,E-cadherin mRNA相对表达水平分别为0.12±0.01和1.53±0.10,α-SMA mRNA相对表达水平分别为3.11±0.24和1.39±0.10,差异均有统计学意义(均P<0.05)。结论宫颈上皮永生化细胞中miR-579-3p mRNA的表达显著高于宫颈癌细胞,miR-579-3p过表达抑制宫颈癌细胞的增殖、侵袭和迁移能力,miR-579-3p可能是潜在的宫颈癌抑癌因子。

Objective To investigate the expression of miR-579-3p in cervical cancer HeLa cells and its effect on the proliferation,migration and invasion of cervical cancer cells and its potential mechanism.Methods The cells were divided into H8 group[normal culture normal cervical epithelial immortalized cells(H8 cells)],HeLa group[normal culture cervical cancer cells(HeLa cells)],HeLa+mimic-NC group(HeLa cells transfected mimic-NC)and HeLa+mimic-miR-579 group(HeLa cells transfected mimic-miR-579-3p).Cell proliferation was detected by cell counting kit-8.Quantitative real-time polymerase chain reaction was used to detect the mRNA expression of miR-579-3p and epithelial-mesenchymal transition(EMT)-related genes.Transwell was used to detect cell migration and invasion.Re su lts The mRNA expression level of miR-579-3p in H8 group(1.00±0.04)was significantly higher than that in HeLa group(0.12±0.01,P<0.001).The relative expression levels of miR-579-3p mRNA in HeLa+mimic-NC group and HeLa+mimic-miR-579 group were 0.13±0.01 and 0.83±0.10;the proliferative capacities on day 2 were 165.91±9.46 and 111.35±9.66;the number of cells invaded was 124.29±11.22 and 48.29±6.58;the number of migrated cells was 83.86±7.22 and 47.86±6.26;the relative expression levels of Viementin mRNA were 3.24±0.38 and 1.49±0.15;the relative expression levels of E-cadherin mRNA were 0.12±0.01 and 1.53±0.10;the relative expression levels ofα-SMA mRNA were 3.11±0.24 and 1.39±0.10,the differences of above indexes were statistically significant(all P<0.05).Conclusion The mRNA expression of miR-579-3p in immortalized cervical epithelial cells is significantly higher than that in cervical cancer cells.Overexpression of miR-579-3p inhibits the proliferation,invasion and migration of cervical cancer cells,and miR-579-3p may be a potential cancer suppressor factor of cervical cancer.