南京地区成人上呼吸道感染患者鼻病毒分子流行病学特征分析

Molecular epidemiological characteristics of human rhinovirus in adults with upper respiratory tract infection in Nanjing

目的分析南京地区成人上呼吸道感染(upper respiratory tract infection,URTI)患者人鼻病毒(human rhinovirus,HRV)的感染特征及流行特征。方法收集2021年10月—2022年9月南京地区成人上呼吸道感染患者的流行病学资料,采集临床标本使用荧光定量逆转录聚合酶链反应开展14种常见呼吸道病毒的检测;对HRV阳性标本VP4/VP2编码区基因扩增测序并做进化分析。结果共收集399份上呼吸道感染患者的咽拭子样本,呼吸道病毒的总体阳性检出率为28.07%(112/399),其中HRV的检出率最高,达到9.52%(38/399);38株HRV阳性标本中37株成功测序,共鉴定为3种基因型,分别为13株HRV-A、14株HRV-B、10株HRV-C,涉及25种血清型;其中3种HRV的基因型呈现交替流行或共流行。结论HRV是2021年10月—2022年9月南京地区成人上呼吸道感染者的主要病原体,且HRV-A、B、C三种基因型共流行或交替流行。

Objective To analyze the clinical and epidemiological features of human rhinovirus(HRV)infection in adult patients with upper respiratory tract infection(URTI)in Nanjing.Methods Epidemiological data of adult patients with URTI in Nanjing from October 2021 to September 2022 were collected.Clinical specimens were collected and subjected to quantitative reverse transcription polymerase chain reaction(qRT-PCR)for the detection of 14 common respiratory viruses.The VP4/VP2 genes in HRV-positive samples were amplified and sequenced.Then a phylogenetic tree was constructed.Results A total of 399 pharyngeal swabs were collected from patients with URTI.The overall positive rate of respiratory viruses was 28.07%(112/399)with HRV accounting for most at 9.52%(38/399).Thirty-seven VP4/VP2 sequences were successfully obtained from the 38 HRV-positive specimens.Three genotypes involving 25 serotypes were identified with 13 strains belonging to HRV-A,14 belonging to HRV-B,and 10 belonging to HRV-C.The three genotypes of HRV showed alternate prevalence or co-prevalence.Conclusions HRV was the main pathogen causing URTI in adult patients in Nanjing from October 2021 to September 2022,and three genotypes of HRV-A,B and C were prevalent alternatively or together.