双补丸干预雷公藤多苷致生殖损伤的作用及代谢组学分析

Metabolomic analysis of the effects of Shuangbu Pill on reproductive damage caused by tripterygium glycosides

目的 观察双补丸(熟地黄、菟丝子等量)干预雷公藤多苷致生殖损伤大鼠的作用,并应用代谢组学方法探讨其调控机制。方法 采用随机数字表法,将21只SPF级4周龄雄性SD大鼠随机分为正常组、雷公藤多苷组(12.5 mg/kg)和双补丸组(3.125 g/kg),每组7只,连续灌胃12周。采用酶联免疫吸附测定法检测大鼠血清睾酮(T)、卵泡生成素(FSH)、促黄体生成素(LH)、雌二醇(E2)水平;全自动精子质量分析仪检测大鼠附睾精子密度及精子活力;HE染色法观察大鼠睾丸、附睾组织病理改变;采用超高效液相色谱-串联飞行时间质谱联用技术结合多元统计方法进行睾丸组织代谢物检测及鉴定,并应用Metabo Analyst平台进行代谢通路分析。结果 与雷公藤多苷组比较,双补丸组大鼠血清T水平升高(P<0.01),精子密度及精子活力提高(P<0.01),睾丸组织生精细胞数量增加,生精细胞空洞化减少,附睾精子数量提高。代谢组学结果表明,双补丸干预后可显著回调19个与雷公藤多苷生殖损伤相关的差异代谢物,其主要涉及谷胱甘肽代谢及甘油磷脂代谢。结论 双补丸可减轻雷公藤多苷引起的雄性大鼠生殖损伤,其作用机制可能是通过调节谷胱甘肽代谢、甘油磷脂代谢实现的。

Objective We aimed to(i)observe the effects of Shuangbu Pill(a compound prescription of prepared Rehmannia root and dodder seed)on reproductive damage induced by tripterygium glycosides in rats and(ii)explore its regulatory mechanism by metabolomic analysis.Methods Using the random number table method,21 male 4-week-old Sprague-Dawley rats were randomly divided into the normal group,the tripterygium glycosides group(12.5 mg/kg),and the Shuangbu Pill group(3.125 g/kg)(7 rats per group)and gavaged continuously for 12 weeks.The serum levels of testosterone(T),follicle stimulating hormone(FSH),luteinizing hormone(LH),and estradiol(E2)were measured by ELISA;sperm density and sperm motility were measured by an automatic sperm quality analyzer;histopathological changes of the testis and epididymis were observed by HE staining;ultrahigh-performance liquid chromatography coupled with tandem time-of-flight mass spectrometry combined with multivariate statistical method was used to detect and identify testicular tissue metabolites;and the Metabo Analyst platform was used for metabolic pathway analysis.Results Compared with the tripterygium glycosides group, the Shuangbu Pill could increase serum T levels (P <0. 01), improve the sperm density andsperm motility ( P < 0. 01), increase the number of spermatogenic cells in testicular tissue, reducespermatogenic cell cavitation, and increase the number of epididymal sperm. According to ourmetabonomic analysis, after treatment with Shuangbu Pill, the levels of 19 differential metabolites relatedto reproductive damage caused by tripterygium glycosides could be significantly restored, which aremainly involved in glutathione metabolism and glycerophospholipid metabolism. Conclusion ShuangbuPill can significantly reduce the reproductive damage caused by tripterygium glycosides, possibly throughregulating glutathione metabolism and glycerophospholipid metabolism.