CEACAM6、miR-146b、S100A6在甲状腺癌中的表达及意义分析

Expression and significance analysis of CEACAM6,miR-146b and S100A6 in thyroid cancer

目的探讨甲状腺癌组织中癌胚抗原相关细胞黏附分子6(carcinoembryonic antigen related cell adhesion molecule 6,CEACAM6)、miR-146b、钙结合蛋白A6(calcium-binding protein A6,S100A6)表达与临床病理特征以及预后的关系。方法选择我院收治的行甲状腺切除手术治疗的甲状腺癌患者165例,分别取癌组织和癌旁组织,采用实时荧光定量聚合酶链反应(real-time fluorescence quantitative polymerase chain reaction,RT-PCR)检测CEACAM6、miR-146b、S100A6表达。采用t检验甲状腺癌患者不同临床病理特征间CEACAM6、miR-146b、S100A6表达差异,Kaplan-Meier和Cox比例风险模型分析CEACAM6、miR-146b、S100A6表达与甲状腺癌患者预后的关系。结果癌组织CEACAM6、miR-146b、S100A6表达高于癌旁组织(P<0.05),肿瘤直径>1 cm、低中度分化、T_(3)~T_(4b)期、N_(1a)~N_(1b)患者癌组织中CEACAM6、miR-146b、S100A6表达高于肿瘤直径≤1 cm、高度分化、T_(1)~T_(2)期、N 0患者(P<0.05)。CEACAM6高表达组、miR-146b高表达组、S100A6高表达组患者总生存率低于CEACAM6低表达组、miR-146b低表达组、S100A6低表达组(P<0.05)。N_(1a)~N_(1b)、CEACAM6高表达、miR-146b高表达、S100A6高表达是甲状腺癌患者不良预后的危险因素(P<0.05)。结论甲状腺癌组织中CEACAM6、miR-146b、S100A6均呈高表达,且与甲状腺癌肿瘤临床病理特征以及预后不良有关。

Objective To investigate the relationship between the expression of carcinoembryonic antigen related cell adhesion molecule 6(CEACAM6),miR-146b and calcium-binding protein A6(S100A6)and clinicopathological features and prognosis in thyroid cancer.Methods A total of 165 thyroid cancer patients who underwent thyroidectomy in our hospital were selected.The expressions of CEACAM6,miR-146b and S100A6 were detected by real-time fluorescence quantitative polymerase chain reaction(RT-PCR)in cancer tissues and adjacent tissues,respectively.A t-test was used to examine the expression differences of CEACAM6,miR-146b and S100A6 among different clinicopathological features of thyroid cancer patients.Kaplan-Meier and Cox proportional risk models were used to analyze the relationship between CEACAM6,miR-146b,S100A6 expression and prognosis of thyroid cancer patients.Results The expressions of CEACAM6,miR-146b and S100A6 in cancer tissues were higher than those in adjacent tissues(P<0.05).The expressions of CEACAM6,miR-146b and S100A6 in patients with tumor diameter>1 cm,low differentiation,T_(3)-T_(4b) and N_(1a)-N_(1b) were higher than those in patients with tumor diameter≤1 cm,high differentiation,T_(1)-T_(2) and N0(P<0.05).The overall survival rate of patients with high expression of CEACAM6,miR-146b and S100A6 were lower than those with low expression of CEACAM6,miR-146b and S100A6(P<0.05).High expression of N_(1a)-N_(1b),CEACAM6,miR-146b and S100A6 were risk factors for poor prognosis of thyroid cancer patients(P<0.05).Conclusion CEACAM6,miR-146b and S100A6 are highly expressed in thyroid cancer tissues,which are related to the clinicopathological features and poor prognosis of thyroid cancer.