不同时段血清尿苷水平与代谢综合征的相关性

Relationship between serum uridine level in different periods and metabolic syndrome

目的利用高效液相色谱质谱联用法(LC⁃MS/MS)检测不同时段血清尿苷水平并探讨其与代谢综合征(MS)的关系。方法选取2021年1月~12月在武汉大学人民医院内分泌科住院的MS患者107例(MS组)及同期体检时发现有甲状腺结节者68例(非MS组),收集两组受试者一般临床资料及实验室检查指标并分组进行比较。采用多元逐步回归分析评估MS的相关影响因素。采用Spearman相关分析评估血清尿苷水平与MS相关指标的相关性。结果MS组男性患者比例、腰围、BMI、肌酐(Cr)、尿酸(UA)、空腹C肽(CpS⁃0h)、餐后2 h C肽(CpS⁃2h)、胰岛素抵抗指数(HOMA⁃IR)及甘油三酯(TG)均高于非MS组,估算的肾小球滤过率(eGFR)及高密度脂蛋白胆固醇(HDL⁃C)均低于非MS组(P<0.05)。MS组空腹血清尿苷水平与非MS组比较差异无统计学意义(P=0.245),餐后血清尿苷水平显著低于非MS组(P=0.002)。多元逐步回归分析结果显示,BMI、TG、Cr为MS发生的相关因素(P<0.01)。Spearman相关分析结果显示,餐后血清尿苷与HOMA⁃IR呈负相关(r=-0.23,P=0.004)。结论餐后血清尿苷不是MS的生物标志物,其可能通过间接影响其他代谢性指标而与MS相关。

Objective Liquid Chromatography⁃tandem Mass Spectrometry(LC⁃MS/MS)was used to detect serum uridine level in different periods and to explore its relationship with metabolic syndrome(MS).Methods A total of 107 patients with MS who were hospitalized in the Department of Endocrinology,Renmin Hospital of Wuhan University from January to December 2021 were selected as MS group,and 68 patients with thyroid nodules in the physical examination during the same period were selected as non⁃MS group.The general clinical data and laboratory examination indexes of the two groups were collected and compared.Multiple stepwise regression analysis was used to evaluate the related factors of MS.Spearman correlation analysis was used to evaluate the correlation between serum uridine levels and MS related index.Results The proportion of male subjects,waist circumference,BMI,UA,Cr,Fasting C⁃Peptide(CpS⁃0h),Postprandial C⁃Peptide(CpS⁃2h),Homeostasis Model Assessment of Insulin Resistance(HOMA⁃IR)and TG in MS group were higher than those in non⁃MS group,and Estimate Glomerular Filtration Rate(eGFR)and HDL⁃C were lower than those in non⁃MS group(P<0.05).There was no significant difference in fasting serum uridine level between MS group and non⁃MS group(P=0.245).The level of postprandial uridine in MS group was lower than that in non⁃MS group(P=0.002).Multiple stepwise regression analysis showed that BMI,TG and Cr were the related factors of MS(P<0.01).Spearman correlation analysis showed that postprandial uridine was negatively correlated with HOMA⁃IR(r=-0.23,P=0.004).Conclusion Postprandial uridine is not a biomarker of MS,and may be associated with MS through indirect effects on other metabolic indicators.