XRCC1在卵子和早期胚胎中的定位与功能的初步分析

Preliminary Study on the Location and Function of XRCC1 in Oocyte and Early Embryos

X射线修复交叉互补蛋白1(XRCC1)是一种支架蛋白,其在碱基切除修复中发挥重要作用。本文检测了XRCC1在卵母细胞和胚胎中的亚细胞定位,并研究了其对小鼠卵母细胞减数分裂成熟的影响,以及对随后的胚胎发育和基因组DNA甲基化的影响。结果显示,在卵母细胞成熟和受精后胚胎发育过程中,XRCC1主要定位在细胞核中。通过显微注射特异性抗体来阻断XRCC1后,卵母细胞生发泡破裂率和胚胎卵裂率出现显著性下降,同时2细胞胚胎中基因组DNA去甲基化水平异常。这说明XRCC1主要定位于细胞周期间期的细胞核,功能正常的XRCC1有助于减数分裂中小鼠卵母细胞质量的维持和早期胚胎的正常发育。

XRCC1 is a molecular scaffold protein that plays a central role in the base excision repair pathway.Here,we examined the subcellular localization of XRCC1 and investigated its effects on meiotic maturation of oocytes,subsequent embryonic development,and global DNA methylation in mice.XRCC1 was found to be localized mainly in the nuclei during oocyte maturation and subsequent embryonic development.Blocking XRCC1 by microinjection with antibodies partly reduced oocyte germinal vesicle breakdown(GVBD)and embryo cleavage,and also disrupts DNA demethylation in the 2-cell embryos.The results indicates that XRCC1 is mainly located in the nuclei at the interphase of cell cycle,and XRCC1 with normal function contributes to the maintenance of the quality of mouse oocytes during meiosis and the normal development of early embryos.