超声引导下微波消融治疗甲状腺微小乳头状癌后患者基因表达变化

Changes of gene expression in patients after ultrasound-guidedmicrowave ablation of papillary thyroid microcarcinoma

目的 观察超声引导下微波消融(MWA)治疗甲状腺微小乳头状癌(PTMC)后患者基因表达变化。方法 前瞻性纳入8例PTMC患者,行超声引导下MWA;分别于MWA前即刻、MWA后1天及1个月抽取其外周静脉血,分离外周血单个核细胞(PBMC),提取mRNA,测序并检测基因表达水平,观察MWA前后基因表达差异,分析相关变化在抗肿瘤免疫中的作用。结果 与MWA前相比,MWA后1天基因表达未见明显差异;MWA后1个月381个基因表达发生变化,2个基因表达上调、379个下调。对379个表达下调基因的生物过程GO功能注释分析结果显示,其中38个基因与免疫反应相关,且大部分编码蛋白具有促炎和促肿瘤作用。结论 超声引导下MWA治疗PTMC后1个月,部分具有促炎和促肿瘤作用的基因表达发生下调。

Objective To observe the changes of gene expression in patients after ultrasound-guided microwave ablation(MWA)of papillary thyroid microcarcinoma(PTMC).Methods Eight PTMC patients were prospectively enrolled,and ultrasound-guided MWA of PTMC was performed.Peripheral venous blood was obtained before MWA,1 day and 1 month after MWA,respectively.Peripheral blood mononuclear cells(PBMC)were isolated,mRNA was extracted and sequenced,and gene expression level was tested.Then the gene expression levels were compared,and the role of relative changes for anti-tumor immunity were analyzed.Results Compared with that before MWA,no significant changes of gene express levels was found 1 day after MWA,while the expression level of 381 genes changed 1 month after MWA,including up-regulated expression levels of 2 genes and down-regulated expression levels of 379 genes.GO function annotation analysis of 379 down-regulated expression genes in biological process showed that 38 genes were related to immune response,and most of the encoded proteins had pro-inflammatory and tumor-promoting effects.Conclusion One month after ultrasound-guided MWA of PTMC,down-regulation of expression level of some genes with pro-inflammatory and tumor-promoting effects occurred.