基于HPLC-QAMS法多成分含量测定结合化学计量学评价半夏天麻丸质量的研究

Quality evaluation of Banxia Tianma Pill by multi-component content determination using HPLC-QAMS and chemometrics

目的建立多指标结合化学计量学综合分析半夏天麻丸成分质量及关键指标的方法,为其质量评价提供科学依据。方法采用高效液相色谱一测多评(high performance liquid chromatography-quantitative analysis of multi-components by single-marker,HPLC-QAMS)法建立12批半夏天麻丸11种成分含量测定方法,运用SPSS 26.0和SIMCA 14.0软件对以上指标进行聚类分析(clustering analysis,CA)、主成分分析(principal component analysis,PCA)及偏最小二乘-判别分析(partial least squares-discriminant analysis,PLS-DA),通过对12批产品的分析,综合评价半夏天麻丸质量及标志性成分。结果建立的11种成分含量测定方法稳定性较好。CA分析可将12批样品聚为三大类,2个主成分可代表11种成分92.142%的信息量,可用2个主成分对半夏天麻丸进行综合评价。根据PLS-DA结果分析,造成其质量差异的指标为橙皮苷、天麻素、柚皮芸香苷、毛蕊异黄酮葡萄糖苷和巴利森苷,可能是影响半夏天麻丸产品质量的差异性标志物。结论本实验首次建立了半夏天麻丸多指标成分质控方法,该方法易操作,重复性与稳定性良好;化学计量学手段也为半夏天麻丸质量控制研究提供了参考依据。

Objective To establish a method for the quality and key indexes of Banxia Tianma Pill(BXTMP)by multi-index combined with chemometrics to provide a scientific basis for its quality evaluation.Methods High performance liquid chromatography-quantitative analysis of multi-components by single-marker(HPLC-QAMS)was used to establish a method for the determination of 11 components content in 12 batches of BXTMP.The quality and iconic components of 12 batches of BXTMP were determined and analyzed by SPSS 26.0 and SIMCA 14.1 software for clustering analysis(CA),principal component analysis(PCA),and partial least squares-discriminant analysis(PLS-DA).Results The determination methods of 11 components were stable.The results showed that the 12-batch samples were classified into three categories by CA,two principal components could account for 92.142%of the information content of 11 components,and two principal components could be used for the comprehensive evaluation of BXTMP.According to PLS-DA,the quality differences were caused by hesperidin,gastrodin,naringin,mullein isoflavone glucoside and parishin.These components may be the differential markers affecting the quality of BXTMP.Conclusion The quality control method of multi-index components of BXTMP was established for the first time.The method is easy to operate,with good repeatability and stability.Chemometrics also provides a reference for the quality control of BXTMP.