禾谷镰刀菌内吞蛋白FgSyp1的功能研究

Functional characterization of endocytic protein FgSyp1 in Fusarium graminearum

内吞作用在细胞的营养吸收和信号转导过程中起重要作用。在真核生物中Syp1和Ede1是网格蛋白介导内吞的早期蛋白成员。禾谷镰刀菌(Fusarium graminearum)引起的小麦赤霉病严重影响小麦产量和品质。禾谷镰刀菌含有酿酒酵母SYP1的一个同源基因FgSYP1。酵母双杂交表明禾谷镰刀菌FgSyp1与FgEde1存在互作关系。利用同源重组的方法得到ΔSyp1突变体和ΔΔSyp1/Ede1双敲突变体,通过生长、产孢和致病性等实验测定禾谷镰刀菌FgSyp1功能。研究表明FgSyp1在禾谷镰刀菌菌丝生长、无性生殖和致病性发挥重要作用,ΔΔSyp1/Ede1双敲突变体在无性和有性生殖方面表现出比ΔSyp1突变体更显著的效果。

Endocytosis plays critical roles in cellular processes of nutrient uptake and signal transduction.Syp1 and Ede1 are early proteins in clathrin-mediated endocytosis.Fusarium head blight mainly caused by Fusarium graminearum seriously affects the yield and quality of wheat.F.graminearum contains one FgSYP1 that is highly homologous to Saccharomyces cerevisiae SYP1.Yeast two-hybrid showed that FgSyp1 interacted with F.graminearum FgEde1.TheΔSyp1 mutant and theΔΔSyp1/Ede1 double mutant were obtained by homologous recombination,and the function of FgSyp1 was determined by experiments including hyphal growth,conidiation and pathogenicity.The results showed that FgSyp1 played an important role in hyphal growth,conidiation and pathogenicity of F.graminearum,and that theΔΔSyp1/Ede1 mutant exhibited obvious defects in asexual and sexual reproduction compared with theΔSyp1 mutant.