摘要
目的:探究DDX5和DDX17在卵巢癌患者组织中的表达及临床意义。方法:采用实时荧光定量PCR检测卵巢癌组织、正常卵巢上皮细胞中DDX5和DDX17的相对表达量,进一步通过基因沉默技术,分别降低DDX5和DDX17的表达后,再采用CCK-8实验测定DDX5和DDX17对卵巢癌细胞增殖的影响。结果:卵巢癌组织中DDX5表达明显高于正常卵巢上皮细胞,而卵巢癌组织中DDX17表达明显低于正常卵巢上皮细胞(P<0.05);沉默DDX5表达后,卵巢癌细胞的增殖活性受到抑制。结论:DDX5和DDX17在卵巢癌组织异常表达,DDX5与卵巢癌细胞的增殖相关。
Objective:To investigate the expression and clinical significance of DDX5 and DDX17 in the tissues of patients with ovarian cancer.Methods:Real-time quantitative PCR was used to detect the relative expression of DDX5 and DDX17 in ovarian cancer tissue and normal ovarian epithelial cells.After further reducing the expression of DDX5 and DDX17 by gene silencing technology,the CCK-8 assay was used to determine the effects of ovarian cancer cell proliferation of DDX5 and DDX17.Results:The expression of DDX5 in ovarian cancer tissue was significantly higher than that in normal ovarian epithelial cells(P<0.05),and the expression of DDX17 in ovarian cancer tissue was significantly lower than that in normal ovarian epithelial cells(P<0.05).After silencing DDX5 expression,the proliferative activity of ovarian cancer cells was suppressed.Conclusion:DDX5 and DDX17 are abnormally expressed in ovarian cancer tissues,and DDX5 is related to the proliferation of ovarian cancer cells.
作者
朱国胜
邱丽影
资捷
ZHU Guosheng;QIU Liying;ZI Jie(Laboratory Department,Maternal and Child Health Hospital,Futian District,Shenzhen City,Guangdong Province 518045)
出处
《医学理论与实践》
2023年第10期1633-1635,1628,共4页
The Journal of Medical Theory and Practice
基金
深圳市福田区卫生公益性科研项目(FTWS2020038)。
