255份马铃薯种质晚疫病广谱Rpi基因标记检测及抗性田间验证

Detection of Late Blight Broad-spectrum Rpi Gene Markers and Field Verification of Resistance in 255 Potato Germplasms

马铃薯(Solanum tuberosum L.)是云南省的第三大粮食作物,晚疫病严重威胁马铃薯的生产,防治晚疫病最好的策略是种植抗性品种。本研究对255份马铃薯种质检测已报道的6个广谱Rpi基因分子标记,并鉴定田间晚疫病抗性。结果表明,含R8、RB、Rpi-blb2、Rpi-sto1、Rpi-sto1、Rpi-vnt1.1基因的材料分别有69、53、51、12、23、75份;81份材料聚合了多个广谱Rpi基因,其中77份来源于国际马铃薯中心(CIP)。田间抗性鉴定结果表明:255份材料中,高抗、中抗、中感和高感材料分别有67、31、67、90份,高抗与中抗材料分别有58份和29份源于CIP。国内种质与CIP种质相比,缺乏抗病与聚合多个广谱Rpi基因的种质。结果还表明,R8基因在晚疫病田间抗性中起主要贡献,R8分子标记田间抗性符合程度较高,并在聚合其他广谱Rpi基因时,符合程度与抗性会随之提高。本研究检测了255份材料的6个广谱Rpi基因分子标记组成,筛选出一批抗性材料,证明了R8分子标记具有较高的晚疫病田间抗性符合程度,可用于选择抗性材料或单株,能为抗病育种与分子标记辅助选择育种提供科学依据。

Potato(Solanum tuberosum L.)is the third most important food crop in Yunnan province,China.Late blight has always been a devastating disease in potato production.Use of resistant varieties is the most fundamental and effective way to control the disease.In this study,screening of broad-spectra Rpi genes using molecular markers and evaluation of late blight resistance upon field inoculation were conduced in 255 potato genotypes.The results showed that 69,53,51,12,23 and 75 varieties(lines)were detected with resistance genes R8,RB,Rpi-blb2,Rpi-sto1,Rpi-sto1 and Rpi-vnt1.1,respectively.Eighty-one of the varieties(lines)were found stacking multiple Rpi genes,and 77 of them were collected from CIP.Gained from field resistance tests,67,31,67 and 90 varieties(lines)were observed with high resistance,medium resistance,medium sensitivity and high sensitivity,respectively.The domestic germplasm was found with lower resistance and rare cases on stacking multiple Rpi genes.The gene R8 largely contributed to the field resistance against late blight.R8 via stacking with other Rpi genes can improve the degree of resistance compliance and field resistance.This study demonstrated that R8 has a high degree of compatibility with field resistance to late blight and could be used for marker assisted selection, which has implication for the future popularization and application of new varieties (lines) and resistance breeding in Yunnan province.