沟槽拓扑基底形貌对宫颈癌HeLa细胞迁移行为的影响

Effects of Groove Topography on Migration of Cervical Cancer HeLa Cells

目的探究沟槽拓扑基底形貌对宫颈癌HeLa细胞形态、迁移速率的影响。方法在4种不同表面特征(平面、槽宽10μm平行沟槽、槽宽20μm平行沟槽、分叉沟槽)PDMS基底上培养HeLa细胞,采用免疫荧光技术对HeLa细胞转染F-actin,并用特异性探针标记线粒体,通过活细胞工作站获取细胞在不同时刻的位置、形态、线粒体分布。结果槽宽10μm平行沟槽中HeLa细胞较槽宽20μm平行沟槽和平面基底排列更加有序,形态更加细长,但平均迁移速率更低;分叉口处HeLa细胞向分叉结构中伸出突出,线粒体主要分布在突出处和细胞核周围,分叉口的存在降低了槽宽10μm平行沟槽中HeLa细胞的平均迁移速率。结论沟槽拓扑基底形貌对HeLa细胞的形态和迁移速率有明显影响。研究结果有助于了解体内微环境中拓扑结构在影响HeLa细胞迁移过程中的作用,并为后续关于宫颈癌侵袭与转移的研究提供参考。

Objective To investigate the effects of groove topography on morphology and migration speed of cervical cancer HeLa cells.Methods HeLa cells were cultured on PDMS substrates with four different surface features,namely,flat substrate,10μm width parallel groove,20μm width parallel groove,bifurcate groove.Immunofluorescence technique was used to transfect F-actin in HeLa cells,and specific probes Mito-Tracker Green were used to label mitochondria.The location,morphology of cells and distribution of mitochondrial at different moments were obtained through the living cell system.Results Compared with 20μm width parallel groove and flat substrate,HeLa cells in 10μm width parallel groove were more orderly arranged and more elongated,but their migration speed was much slower.HeLa cells at the bifurcation protruded into branches and mitochondria were mainly distributed at the protrusion and around the nucleus.The bifurcation reduced the average migration speed of HeLa cells in 10μm width parallel groove.Conclusions Groove topography has a significant effect on morphology and migration speed of HeLa cells.The research findings help to understand the role of topography in in vivo microenvironment during migration of HeLa cells,and provide references for the subsequent research on invasion and metastasis of cervical cancers.