贝壳珍珠质及交叉叠片结构体外类骨磷灰石生长的对比研究

Comparative Investigations of in vitro Apatites Deposition in Nacre and Crossed-lamellar Structures in Mollusk Shells

对具有交叉叠片结构的扇贝和珍珠质结构的褶纹冠蚌贝壳进行表面预处理和体外生物活性实验,对比研究了类骨磷灰石在这两种不同微观结构表面的生长。在磷酸缓冲液PBS中浸泡的初始阶段,磷灰石在交错层状结构表面沉积得很紧密,沉积速度较高;而在SBF模拟人体体液中的体外矿化过程中,在交叉叠片结构表面在较短时间内就生成了数量更多的磷灰石。长期浸泡后,两种结构的生物活性没有明显的不同。与珍珠质结构相比,在短时间内交叉叠片结构更有利于类骨磷灰石的生长。

One specie of Pectinidae with a crossed-lamellar structure and one of Plicata with a nacreous structure were selected as the target materials in this paper.The growth of bone-like apatite on the surfaces of these two kinds of microstructures by a pre-treatment and an in vitro bioactivity assessment was investigated.The apatites were deposited more compactly and more quickly on the surface of crossed-lamellar structure in the preliminary stage of immersion in PBS,as well as in SBF.However,these two kinds of microstructures exhibit identically an excellent bioactivity after a subsequently longterm in vitro mineralization process in SBF.Therefore,in contrast to nacreous structure the crossed-lamellar structure can promote the deposition of the apatites no matter in a short term or in a long term.