番茄WRKY转录因子比较鉴定及细菌胁迫响应

Identification and Pathogenic Response of Tomato WRKY Transcription Factors

【目的】深入了解番茄WRKY转录因子的组学特征及其生物胁迫响应。【方法】基于最新公共数据,利用生物信息学和比较基因组学方法对番茄WRKY进行系统鉴定,结合抗、感2个番茄自交系在青枯菌侵染前后的RNA-seq数据,挖掘青枯病抗性相关WRKY。【结果】85个番茄WRKY转录因子被鉴定,可分为Ⅰ、Ⅱa+b、Ⅱc、Ⅱd+e和Ⅲ等类别,Ⅱe基因最多。其中,9个基因七肽基序发生了单一氨基酸变异,WRKYGKK为优势突变型。这些WRKY主要分布在5号染色体,且具有端部和成簇分布现象,尤其是Ⅱe亚类。45.88%的番茄WRKY具有共线性。58.82%的番茄WRKY(主要是Ⅰ和Ⅱc类)与拟南芥和辣椒WRKY形成73对直系同源基因,其选择压力(Ka/Ks)均小于1。16个番茄WRKY(主要是Ⅱa+b和Ⅱc类)对几种生物胁迫反应强烈,且主要在根中表达。12个差异表达WRKY(主要是Ⅲ和Ⅱb类)被鉴定,其中Solyc03g095770.3(Ⅲ)与Solyc09g014990.4(Ⅰ)互作在番茄青枯病响应中发挥重要作用。【结论】综合鉴定了番茄WRKY转录因子,筛选到12个青枯病响应基因。

【Objective】Characteristics and biotic stress response of WRKY transcription factors(TFs)in tomato plants were investigated.【Method】The latest available bioinformatics and genomics methods were employed to identify the tomato WRKY TFs.RNA-seq of disease-resistant and susceptible tomato inbred lines before and after artificial Ralstonia solanacearum infection were obtained to identify the TFs associated with the pathogenic resistance of the plants.【Result】Eighty-five tomato WRKY TFs were identified and divided into I,IIa+b,IIc,IId+e,and III categories.The IIe group had the highest number of the TFs.The conserved motif of 9 TFs had one single amino acid variation,and WRKYGKK was the dominant mutant.The TFs,especially those in the IIe group,were mainly found on chromosome 5,at the ends,and in clusters.In them,45.88%showed collinearity and 58.82%(mainly in I and IIc groups)formed 73 pairs of orthologs with those in Arabidopsis and chili pepper at a Ka/Ks ratio below 1.Sixteen of them,mainly belonging to IIa+b and IIc,responded significantly to the biotic stress with expressions largely in the roots.There were 12 differentially expressed WRKY TFs identified mainly in III and IIb.Of which,the interaction between Solyc03g095770.3(III)and Solyc09g014990.4(I)played a significant role in the response of the tomato plant to bacterial wilt.【Conclusion】The WRKY TFs were identified in tomato plants.Twelve genes responded to the bacterial wilt were isolated.