大白菜F-BOX基因家族的鉴定与核盘菌诱导应答分析

Genome-wide Identification and Transcript Response to Sclerotinia of F-BOX Genes in Chinese Cabbage

【目的】筛选参与菌核病应答反应的F-BOX基因,为大白菜抗菌核病基因的功能研究及抗病品种的选育提供基础。【方法】基于核盘菌侵染大白菜转录组测序数据,对大白菜F-BOX基因家族进行鉴定,进行亚细胞定位、染色体定位、保守结构域等生物信息学分析。分析F-BOX基因在核盘菌侵染下的差异表达,并采用q-PCR技术检测F-BOX基因在0 h和36 h的表达情况。【结果】共鉴定32个BraF-BOX基因,分子量介于34 751.13~105 942.22Da;亚细胞定位表明,26个F-BOX基因定位在细胞核和细胞质中,6个定位在叶绿体中;系统进化树分析将BraFBOX分为4个亚族;基因结构分析结果表明,每个BraF-BOX家族成员均含有Motif 1,序列中均含有外显子;组织特异性表达分析结果表明在不同大白菜部位,表达量有差异;在核盘菌处理36 h时,q-PCR检测6个基因的相对表达量趋势与转录组数据一致,其中Bra037120、Bra011427、Bra009835等3个基因表达上调,核盘菌侵染时间越长,表达量越大。【结论】综合BraF-BOX基因生物信息学及转录组数据分析,Bra037120、Bra011427、Bra009835基因可能参与大白菜菌核病抗性功能,为后续基因功能研究提供研究基础。

【Objective】F-BOX genes responsible for the resistance of Brassica rapa to sclerotinia were investigated.【Method】Suspected members of F-BOX family were identified at the transcriptome level to identify their subcellular localization,chromosomal localization,and conserved domains as well as expressions by q-PCR under artificial induction of sclerotinia.【Result】There were 32 BraF-BOX genes classified into 4 subgroups with the relative molecular masses varying from 34751.13 Da to 105942.22 Da.The predicted subcellular localizations of 26 F-BOX were in the nucleus and cytoplasm,and 6 in the chloroplast.The genetic structures of all 32 genes contained Motif 1 and exon in the DNA sequences with expressions differed in different parts of Chinese cabbage.The relative expressions by q-PCR of 6 genes agreed with the transcriptome data at 36 h.The expressions of Bra037120,Bra011427,and Bra009835 were upregulated and increased with prolonged induction.【Conclusion】The bioinformatics and transcriptome data analysis on the BraF-BOX genes suggested that Bra037120,Bra011427,and Bra009835 were potentially associated with the sclerotinia resistance of Chinese cabbage.