基于生物标签研究模式运用组学和生物信息学定位杜仲叶治疗脊髓疾病的潜能、作用机制及物质基础

Positioning Therapeutic Potential,Mechanism of Action and Material Basis of Duzhongye(Eucommiae Folium)in Treatment of Spinal Cord Diseases Based on Biolabel-Led Research Pattern by Applying Omics and Bioinformatics

目的基于生物标签研究模式运用组学和生物信息学定位杜仲叶治疗脊髓疾病的潜能、作用机制及物质基础。方法蛋白质组学和代谢组学被用于探索杜仲叶干预脊髓组织的生物标签,并在此基础上运用生物信息学对其治疗潜能、作用机制和物质基础进行拓扑分析。结果26个差异表达蛋白质和7个差异表达代谢物参与了15个显著调节通路,可以作为潜在生物标签。通过生物信息学数据库的分析,杜仲叶的治疗潜能可能涉及了肌萎缩性脊髓侧索硬化症、脊髓损伤及脊髓小脑共济失调。相关作用机制可能涉及了凋亡、炎症、氧化应激等病理过程。杜仲叶的13个化学成分可能成为其治疗这3种脊髓疾病的共同物质基础。结论基于生物标签研究模式,组学和生物信息学可以用于定位杜仲叶治疗脊髓疾病的潜能、作用机制及物质基础。这可以为杜仲叶后续的研究及临床治疗谱的明确提供重要的参考依据。

Objective To position the therapeutic potential,mechanism of action and material basis of Duzhongye(Eucommiae Folium,EF)in the treatment of spinal cord diseases based on the biolabel-led research pattern by applying omics and bioinformatics.Methods Proteomics and metabonomics were applied to explore the biolabels responsible for the effect of EF on spinal cord tissue,based on which its therapeutic potential,mechanism of action and material basis were topologically analyzed by bioinformatics.Results A total of 26 proteins and 7 metabolites were significantly enriched in 15 pathways and were considered as biolabels.Through the analysis of bioinformatics databases,the therapeutic potential of EF may involve amyotrophic lateral sclerosis,spinal cord injury and spinocerebellar ataxia.The related mechanisms may involve apoptosis,inflammation,oxidative stress and other pathological processes.Thirteen compounds of EF may be the common material basis for the treatment of these spinal cord diseases.Conclusion Omics and bioinformatics can be used to position the therapeutic potential,mechanism of action and material basis of EF in the treatment of spinal cord diseases based on the biolabel-led research pattern.These results provide the important reference for the follow-up studies and clear clinical treatment spectrum of EF.