分光光度法测定油茶籽油中总三萜含量的前处理方法研究

Pretreatment for determination of total triterpene in oil-tea camellia seed oil by spectrophotometry

为准确定量油茶籽油中三萜类物质,对分光光度法测定油茶籽油中总三萜含量的样品前处理方法进行研究。通过单因素试验和正交试验考察了乙醇体积分数、提取温度、提取时间和提取次数对油茶籽油中总三萜含量的影响。结果表明:各因素对油茶籽油中总三萜提取的影响程度从大到小依次为乙醇体积分数>提取次数>提取温度=提取时间;油茶籽油中总三萜的最佳提取工艺条件为乙醇体积分数80%、提取温度80℃、提取时间40 min、提取次数4次,在此条件下提取测得油茶籽油中总三萜含量为(0.78±0.06)%(以齐墩果酸计)。综上,该方法操作简便、快速、灵敏度高,可用于油茶籽油中总三萜含量的测定。

To accurately quantify triterpenoids in oil-tea camellia seed oil, a sample pretreament method was investigated for the determination of total triterpene in oil-tea camellia seed oil by spectrophotometry. The effects of volume fraction of ethanol, extraction temperature, extraction time and extraction times on the extraction of total triterpene from oil-tea camellia seed oil were investigated by single factor experiment and orthogonal experiment. The results showed that the significance of the factors affecting the extraction of total triterpene from oil-tea camellia seed oil decreased in the order: ethanol volume fraction> extraction times> extraction temperature = extraction time. The optimal extraction conditions of total triterpene were as follows: volume fraction of ethanol 80%, extraction temperature 80℃, extraction time 40 min, and extraction times 4. Under these conditions, the content of total triterpene in oil-tea camellia seed oil was (0.78±0.06)% (calculated by oleanolic acid). In conclusion, the method is simple, rapid and sensitive, and can be used for the determination of total triterpene in oil-tea camellia seed oil.