丽江百合组织培养及再生体系建立

Establishment of Tissue Culture and Regeneration System of Lilium lijiangense

为我国特有易危种丽江百合(Lilium lijiangense)的种质资源保护、快速繁殖及园林应用奠定基础,以野生丽江百合鳞片为外植体,探究其最佳灭菌方法,鳞茎不同部位诱导不定芽的能力,以及不同植物生长调节剂对丽江百合愈伤组织及不定芽诱导、不定芽增殖、生根结鳞茎的影响。结果表明,丽江百合鳞片最佳灭菌方案为5%NaClO 8 min+75%乙醇30 s;鳞片分化能力为基部>中部>上部;愈伤组织和不定芽诱导最佳培养基配方为MS+2.0 mg/L 6-BA+0.1 mg/L NAA;不定芽增殖最佳培养基为MS+1.0 mg/L 6-BA+0.2 mg/L NAA;最佳生根结鳞茎培养基为1/2MS+0.1 mg/L NAA+0.01 mg/L IBA。该研究成功建立了一套适宜丽江百合的组织培养和再生体系。

In order to lay a foundation for the germplasm protection,rapid propagation and garden application of Lilium lijiangense,an endemic vulnerable species in China,this paper took the scales of wild L.lijiangense as explants,the best sterilization method,the ability of inducing adventitious buds in different scale parts,and the effects of different plant growth regulators on callus and adventitious bud induction,bud proliferation,rooting and buld proliferation of L.lijiangense were studied.The results were as follows:The best sterilization scheme was 5%NaClO 8 min+75%alcohol 30 s;The differentiation ability of scales was base>middle>upper;The best medium for callus and adventitious bud induction was MS+2.0 mg/L 6-BA+0.1 mg/L NAA;The best medium for adventitious bud proliferation was MS+1.0 mg/L 6-BA+0.2 mg/L NAA;The best rooting and bulb proliferation medium was 1/2MS+0.1mg/L NAA+0.01 mg/L IBA.Overall,a set of tissue culture and regeneration system suitable for L.lijiangense is successfully established.