高效液相色谱法测定玉米中玉米赤霉烯酮

Determination of Zearalenone in Corn by High Performance Liquid Chromatography

本文建立了玉米中玉米赤霉烯酮的高效液相色谱分析方法,用有机溶剂提取样品中的玉米赤霉烯酮,经免疫亲和柱净化后,用高效液相色谱-荧光检测器检测,以保留时间定性,外标法定量。经检测,玉米赤霉烯酮线性范围为10.16~508.00 ng·mL-1,相关系数为0.999 93,回收率为84.0%~92.4%,检出限为0.12 ng·mL-1。结果表明,该方法可以确保玉米赤霉烯酮检测的准确性和稳定性。

This paper develops a method for the determination of zearalenone in corn by high performance liquid chromatography(HPLC).This method uses organic solvent to extract zearalenone,which is purified by immunoaffinity column,and then detected by high performance liquid chromatography-fluorescence detector,qualitative by retention time and quantitative by external standard method.The data showed that the linear range of zearalenone was 10.16~508.00 ng·mL-1,and the correlation coefficient was 0.99993,the range of recovery was 84.0%~92.4%,the detection limit of zearalenone was 0.12 ng·mL-1.The results showed that this method can ensure the accuracy and stability of zearalenone detection results.