基于植物DNA条形码rbcLa序列的日本条螽食谱分析

Dietary analysis of Ducetia japonica based on plant DNA barcoding rbcLa sequences

日本条螽Ducetia japonica广泛分布于我国绝大部分区域,并在城区绿地大量发生。据此假设其食物谱可能具有较高的物种多样性,但目前对其在自然环境下取食的植物种类仍缺乏了解。本研究对来自河北保定城区绿地和城郊荒地两种生境共计75头日本条螽肠道内容物进行DNA提取、rbcLa基因片段扩增及测序,并利用BOLD和NCBI数据库进行分类鉴定。结果显示:日本条螽在野外至少取食10科、13种植物。在城区绿地和城郊荒地两种生境中,取食频率最高的植物分别是卫矛科冬青卫矛Euonymus japonicus(47.37%)和大麻科葎草Humulus scandens(81.08%)。只有1条rbcLa基因序列未鉴定至种级水平,表明利用植物DNA条形码rbcLa基因序列可对植食性昆虫取食的植物种类进行快速鉴定。同时,研究结果也初步证实日本条螽的食物谱包含较高的物种多样性,为城市绿地螽斯类鸣虫保护和繁育提供依据。

Ducetia japonica is widely distributed in the vast majority areas of China,and abundant in urban parks.We hypothesized that it might be related to its broad host range.The natural diets of D.japonica was still unclear.Plant DNA barcoding,using chloroplast ribulose-bisphosphate carboxylase(rbcLa)gene fragment,could be used for dietary analysis of herbivores.In this study,we amplified and sequenced rbcLa sequences from the gut contents of 75 D.japonica specimens from two habitata,urban green space and suburban wasteland.Taxonomic classification and identity assignment was performed using a reference-based approach with the BOLD and NCBI database of rbcLa sequences.The dietary profile of D.japonica consists of at least 13 plant species from 10 families.It apparently preferred Euonymus japonicus(18 out of 38 specimens)belong in Celastraceae in urban green space and Humulus scandens(30 out of 37 specimens)belong in Cannabaceae in suburban wasteland.Only one unidentified species demonstrates that plant DNA barcoding rbcLa sequences could be used to rapidly obtain dietary profile data.Our results also support previous hypothesized that the broad host range of D.japonica was significantly associated with its geographical distribution.