香蕉皮提取物通过调节肠道菌群和肠神经递质水平提高便秘模型小鼠肠动力

Banana peel extract improves intestinal motility of constipation model mice by regulating intestinal flora and intestinal neurotransmitter levels

探究香蕉皮提取物对便秘小鼠肠动力的干预作用。本研究采用混合抗生素溶液构建便秘小鼠模型。用香蕉皮提取物溶液对便秘小鼠进行干预,并测定其对便秘小鼠的肠道菌群、肠神经递质水平和肠动力的影响。结果表明,高浓度香蕉皮提取物组小鼠的粪便含水量增加了137.5%、小肠推进率增加了3.4倍、首粒黑便排出时长减少了73.7%(P<0.05),且基本恢复到空白组小鼠水平。便秘模型小鼠菌群紊乱,部分有益菌缺失,经低浓度香蕉皮提取物干预后,属水平上Trabulsiella(特布尔西菌属)和Unclassified-f-Enterobacteriaceae的相对丰度增加了6倍,肠道Bacteroides(拟杆菌属)的相对丰度减少了94.4%。在神经递质中,低浓度香蕉皮提取物组小鼠的去甲肾上腺素、多巴胺、γ-氨基丁酸、乙酰胆碱的浓度显著增加了6.1%、17.6%、63.7%、52.6%。以上结果表明,香蕉皮提取物可调节肠道菌群和肠神经递质水平提高便秘小鼠肠动力。

To explore the effect of banana peel extract on intestinal motility in constipated mice.In this study,the mice model of constipation was established with the mixed antibiotic solution.The effects of banana peel extract on intestinal flora,intestinal neurotransmitter and intestinal motiveness of constipated mice were measured.The results showed that the fecal water content increased by 137.5%,the intestinal propulsion rate increased by 3.4 times,and the first black stool excretion time decreased by 73.7%(P<0.05)in the high-concentration banana peel extract group,which basically recovered to the level of the blank group.The phyla horizontal flora was disordered and some beneficial bacteria were missing in the constipation model mice.After the intervention of low concentration banana peel extract,the relative abundance of Trabulsiella and Unclassified-f-Enterobacteriaceae increased by 6 times,the relative abundance of intestinal Bacteroides decreased by 94.4%.Furthermore,the concentrations of norepinephrine,dopamine,gamma-aminobutyric acid and acetylcholine of neurotransmitters were significantly increased by 6.1%,17.6%,63.7%and 52.6%.These results indicated that banana peel extract could regulate intestinal flora and intestinal neurotransmitter levels to improve intestinal motility in constipated mice.