地氟烷与七氟烷麻醉对睡眠剥夺小鼠睡眠质量影响的比较

Comparison of effects of desflurane versus sevoflurane anesthesia on sleep quality of sleep-deprived mice

目的比较地氟烷与七氟烷麻醉对睡眠剥夺小鼠睡眠质量的影响。方法清洁级健康雄性C57BL/6小鼠32只,10周龄,体质量20~25 g,采用随机数字表法分为4组(n=8):对照组(C组)、睡眠剥夺组(SD组)、睡眠剥夺+七氟烷组(SD+SEV组)和睡眠剥夺+地氟烷组(SD+DES组)。4组小鼠均进行脑电-肌电电极埋置手术,适应7 d后采用毛刷轻柔刺激法小鼠完全睡眠剥夺12 h(6:00—18:00)。睡眠剥夺后6 h分为2个时间节段:T_(1)期2 h(18:00—20:00)和T_(2)期4 h(20:00—24:00)。T_(1)期:SD组睡眠剥夺后自然睡眠,C组和SD组均吸入60%氧气1.5 L/min。SD+DES组和SD+SEV组睡眠剥夺后暴露于6%地氟烷或2.5%七氟烷2 h,60%氧气1.5 L/min。T_(2)期:4组小鼠均自然睡眠,同时记录脑电-肌电信号,采用Lunion Data软件计算觉醒(WAKE)时间、快动眼睡眠(REM)时间和非快动眼睡眠(NREM)时间占总时间的百分比和次数。结果与C组比较,SD组、SD+SEV组和SD+DES组12 h睡眠剥夺期间,NREM和REM时间百分比降低,WAKE时间百分比升高(P<0.05);与T_(1)期比较,SD组T_(2)期NREM时间百分比升高,WAKE和REM时间百分比降低(P<0.05);与SD组比较,SD+SEV组和SD+DES组T_(2)期NREM和REM时间百分比降低,WAKE时间百分比升高(P<0.05);SD+SEV组和SD+DES组T_(2)期NREM、REM和WAKE时间百分比差异无统计学意义(P>0.05);与SD+SEV组比较,SD+DES组T_(2)期NREM次数减少(P<0.05)。结论地氟烷麻醉改善睡眠剥夺小鼠睡眠质量的效应优于七氟烷麻醉。

Objective To compare the effects of desflurane and sevoflurane anesthesia on the sleep quality of sleep-deprived mice.Methods Thirty-two clean-grade healthy male C57BL/6 mice,aged 10 weeks,weighing 20-25 g,were divided into 4 groups(n=8 each)by the random number table method:control group(C group),sleep deprivation group(SD group),sleep deprivation+sevoflurane group(SD+SEV group),and sleep deprivation+desflurane group(SD+DES group).In the four groups,EEG-EMG electrodes were implanted for recording EEG and EMG,and sleep deprivation model was developed by the gentle stimulation method with a brush for 12 h(6:00-18:00)after 7 days of adaptation.The 6 h after sleep deprivation was divided into 2 time periods:T_(1) period(18:00-20:00)and T_(2) period(20:00-24:00).T_(1) period In SD group,mice were allowed ad libitum recovery sleep after sleep deprivation.C group and SD group were exposed to 60%oxygen 1.5 L/min.In SD+DES group and SD+SEV group,mice were exposed to 6%desflurane and 2.5%sevoflurane,respectively,for 2 h in 60%oxygen 1.5 L/min following sleep deprivation.T_(2) period Four groups were allowed ad libitum recovery sleep with the EEG-EMG signal recording.The percentages and number of wakefulness time,rapid eye movement time and non-rapid eye movement time during each time period were calculated using Lunion Data software.Results Compared with C group,the percentage of non-rapid eye movement time and the percentage of rapid eye movement time were significantly decreased,and the percentage of wakefulness time was increased during 12 h sleep deprivation in SD group,SD+SEV group and SD+DES group(P<0.05).Compared with T_(1) period,the percentage of non-rapid eye movement time was significantly increased,and the percentage of wakefulness time and percentage of rapid eye movement time were decreased in T_(2) period in SD group(P<0.05).Compared with SD group,the percentage of non-rapid eye movement time and percentage of rapid eye movement time were significantly decreased,and the percentage of wakefulness time was increased in T_(2) period in SD+SEV group and SD+DES group(P<0.05).There was no significant difference in the percentage of non-rapid eye movement,rapid eye movement and wakefulness time in T_(2) period between SD+SEV group and SD+DES group(P>0.05).Compared with SD+SEV group,the number of non-rapid eye movement in T_(2) period was significantly reduced in SD+DES group(P<0.05).Conclusions The effect of desflurane anesthesia in improving sleep quality is better than sevoflurane anesthesia in sleep-deprived mice.