生长素与高粱杂种优势关系分析

Study on the relationship between auxin and sorghum heterosis

【目的】研究高粱生长素(IAA)与杂种优势之间的潜在关系,为高粱育种中杂种优势的利用提供重要理论依据。【方法】以11个亲本及组配的11个杂交种为材料,采用高效液相色谱技术测定杂交种及其亲本生长素在抽穗期和成熟期的含量,利用实时荧光定量PCR技术比较抽穗期、成熟期高粱不同品种的生长素基因表达的差异。【结果】11个杂交种成熟期的生长素含量均高于抽穗期,成熟期杂种辽粘3号、辽杂19、辽杂35、辽杂36、辽2297、辽2697、辽5397、辽糯7号、辽糯11号、辽夏梁1号的生长素含量与其亲本相比,子代生长素含量更高,成熟期只有辽粘6号的生长素含量比其亲本的低。以对应的母本或父本为对照,杂交种辽粘3号和辽杂19在抽穗期的生长素基因表达量低于其亲本,杂交种辽粘3号、辽粘6号、辽杂36在成熟期的生长素基因表达量低于其亲本,其余杂交品种在抽穗期、成熟期生长素基因表达量均高于其亲本。【结论】内源生长素是调节高粱生长杂种优势的一个因素,但不是唯一因素。

【Objective】In To explore the potential relationship between sorghum auxin(IAA)and heterosis,and provide an important theoretical basis for the utilization of heterosis in sorghum breeding in the future.【Methods】With 11 parents and 11 hybrids as research materials,the content of auxin in the hybrids and their parents at the heading and maturity stages was determined by high performance liquid chromatography,and the heading dates were compared by real-time fluorescent quantitative PCR technology,which was used again to compare the differences in auxin gene expression of different varieties of sorghum at the heading and maturity stages.【Results】The results showed that the auxin content of the 11 hybrids at the mature stage was higher than that at the heading stage.The auxin content of Liaoning 3,Liaoza 19,Liaoza 35,Liaoza 36,Liao 2297,Liao 2697,Liao 5397,Liaonuo 7,Liaonuo 11,Liaoxialiang 1 and their parents in mature period.In contrast,the progeny had higher auxin content,and only Liaoning 6 had lower auxin content than its parents at maturity.When the corresponding female parent or male parent was taken as the control,the auxin gene expression levels of Liaoning 3 and Liaoza 19 were lower than their parents during the heading stage.At the maturity stage,the auxin gene expression levels of Liaoning 3,Liaoning 6 and Liaoza 36 were lower than their parents.The expression levels of auxin genes in other hybrids were higher than their parents.【Conclusion】The finding shows that endogenous auxin is a factor that regulates the heterosis of sorghum growth,but not the only one.