日粮中添加不同比例葡萄籽对多浪羊十二指肠细菌菌群结构的影响

Effects of different proportion of grape seeds in diet on intestinal bacterial flora structure of Duolang sheep

【目的】研究日粮中添加不同比例葡萄籽对多浪羊肠道细菌菌群结构的影响。【方法】选取25只3月龄体重(20±1.5)kg的健康多浪羊为试验动物,随机分为5组,每组5只羊,单栏饲养。葡萄籽添加比例分别为0%(对照组,A组)、4%(B组)、8%(C组)、12%(D组)、16%(E组)。预饲期15 d,正试期90 d。结束后屠宰,采集十二指肠食糜,利用16S rRNA测序技术分析肠道细菌菌群的结构。【结果】(1)样品测序覆盖率98.22%~98.82%,各组Alpha多样性差异不显著(P>0.05);25个样品测样出现了1038个OTU,统计分析试验组与对照组差异均不显著(P>0.05);(2)门水平优势菌门为厚壁菌门(Firmicutes)与放线菌门(Actinobacteria),A组和B组拟杆菌门(Bacteroidetes)相对丰度与C组、D组、E组相比差异显著(P<0.05)。(3)Aeriscardovia为A、B、C、E四组第一优势菌群,占比最高,而D组第一优势菌群为毛螺菌科NK3A20属(Lachnospiraceae_NK3A20_group)。【结论】日粮中添加不同比例葡萄籽不影响多浪羊肠道细菌多样性,但影响部分菌群丰度。

【Objective】To explore the effects on the structure of intestinal bacterial flora by adding different ratios of grape seeds to the diet of Duolang sheep.【Methods】25 Duolang lambs aged(20±1.5)kg at the age of 3 months were selected as experimental animals.According to the experimental requirements,they were randomly divided into 5 groups,5 in each group in parallel,all of which were fed in a single column.The addition of grape seeds was 0%(control group,group A),4%(group B),8%(Group C),12%(Group D)and 16%(Group E).Pre feeding lasted for 15 days and trial feeding for 90 days.Duodenal chyme was collected after slaughter and the structure of intestinal bacterial flora was analyzed by 16S rRNA sequencing.【Results】(1)The sequencing coverage of 25 samples was 98.22%-98.82%.There was no significant difference in alpha diversity among groups(P>0.05).There were 1,038 OTUs in 25 samples.Statistical analysis showed that there was no significant difference between the experimental group and the control group(P>0.05);(2)The dominant phyla at phylum level were Firmicutes and actinomycetes.The relative abundance of bacteroidetes in group A and group B was significantly higher than those in group C,group D and group E(P<0.05).(3)Aeriscardovia was the first dominant flora in group A,B,C and E,accounting for the highest proportion,while the first dominant flora in group D was NK3A20 genus of spirillaceae.【Conclusion】In conclusion,the addition of different proportions of grape seeds in the diet do not affect the intestinal bacterial diversity of Duolang sheep,but affect the abundance of some flora.