P53、Ki-67蛋白在前列腺癌的表达及其临床意义

Expressions of P53 and Ki-67 in prostate cancer and the clinicopathological significance

目的研究P53和Ki-67蛋白在前列腺癌组织中的表达情况,探讨P53和Ki-67蛋白的表达与前列腺癌临床病理特征之间的关系。方法应用免疫组化法检测90例根治性前列腺癌切除术后组织标本中P53、细胞增殖指标Ki-67的表达情况。记录所有患者年龄、术前血清前列腺特异性抗原(PSA)水平、术后Gleason评分、病理分期、神经血管癌栓浸润情况。分析P53表达与上述各项指标的相关性。结果前列腺癌组织P53和Ki-67表达(≥3%)阳性率分别为27.8%(25/90)和46.7%(42/90)。P53在pT2和pT3~T4期的阳性率为50.0%和19.7%(P=0.005),而Ki-67在pT2和pT3~T4期的阳性率为36.4%和75.0%(P=0.001)。Ki-67在Gleason评分≤6分、7分和≥8分组中表达阳性分别为30.4%、53.8%和66.7%,差异具有统计学意义。P53表达阳性与Ki-67表达相关(P=0.012)。P53表达与年龄、术前血PSA水平、术后Gleason评分、血管神经癌栓浸润差异无统计学意义。结论前列腺癌P53蛋白表达与肿瘤分期和Ki-67相关,而Ki-67表达与肿瘤分期分级均相关。

Objective To investigate the expressions of P53 and Ki-67 in prostate cancer(PCa)and to explore their correlation with the clinicopathological characteristics.Methods The expressions of P53 and Ki-67 in 90 PCa patients were detected with immunohistochemistry.Patients age,preoperative prostate-specific antigen(PSA)level,postoperative Gleason score,pathological stage,and invasion of neurovascular cancer embolus of all patients were recorded.The relationship of P53 expression with the above indexes was evaluated.Results The positive rates of P53 and Ki-67 were 27.8%(25/90)and 46.7%(42/90),respectively.The positive rate of P53 in pT2 and pT3-T4 stage groups were 19.7%(13/66)and 50.0%(12/24)(P=0.005),and the positive rate of Ki-67 were 36.4%(24/66)and 75.0%(18/24)(P=0.001),respectively.The positive rate of Ki-67 in Gleason score≤6,≤7 and≥8 groups were 30.4%,53.8%and 66.7%,respectively,with statistical difference.Positive expression of P53 was related to Ki-67 expression,but not to patients age,preoperative PSA level,postoperative Gleason score and nerve and invasion of neurovascular cancer embolus.Conclusion P53 expression is related to tumor stage and Ki-67,while Ki-67 expression is associated with tumor stage ang grade.