香叶木素对小鼠肾缺血再灌注损伤的保护作用

Protective effects of diosmetin on renal ischemia-reperfusion injury in mice

目的分析香叶木素对小鼠肾缺血再灌注(I/R)损伤是否具有保护作用及其潜在机制。方法将45只BALB/c雄性小鼠分为假手术组、模型组和香叶木素处理组,通过检测血生化指标和肾组织HE染色损伤评分评价香叶木素对小鼠肾功能的保护作用,通过免疫组化和蛋白免疫印迹检测NF-κB家族关键分子p-P65表达水平变化的情况,通过实时荧光定量聚合酶链式反应检测NF-κB信号通路下游炎症因子的变化情况。结果与假手术组比较,模型组小鼠血肌酐和尿素氮水平显著升高,小鼠肾组织损伤显著,p-P65以及下游炎症因子表达显著增加。通过香叶木素处理后,可改善肾功能血生化指标,减轻小鼠肾组织损伤,并发现香叶木素可以下调肾I/R损伤中p-P65和相关炎症因子的表达。结论香叶木素可能通过抑制NF-κB信号通路的激活,从而在小鼠肾I/R损伤中起到保护作用。

Objective To investigate whether diosmetin has protective effects on renal ischemia-reperfusion(I/R)injury in mice and explore the potential mechanism.Methods A total of 45 BALB/c male mice were randomly divided into the sham group,I/R group and diosmetin treatment group.The protective effects of diosmetin on the renal function of mice was evaluated by detecting blood biochemical indexes and renal tissue HE staining injury score.The expression of p-P65,a key molecule of NF-κB family,was detected with immunohistochemistry and western blotting,and the downstream inflammatory factors of NF-κB signaling pathway were detected with quantitative polymerace chain reaction(qPCR).Results Compared with the sham group,the I/R group had significantly increased levels of serum creatinine and urea nitrogen,significantly damaged morphological construction,and significantly increased expressions of p-P65 and inflammatory cytokines,while the diosmetin treatment group had decreased levels of serological indexes,improved structure of kidney tissue and reduced expressions of p-P65 and inflammatory cytokines.Conclusion Diosmetin can protect kidney from I/R-induced acute kidney injury by inhibiting NF-κB pathway in mice.