柳叶蒿地上部分中酚酸类成分的分离与结构鉴定

Isolation and identification of phenolic constituents from the aerial part of Artemisia integrifolia L.

目的对柳叶蒿地上部分的化学成分进行研究。方法应用硅胶、ODS及高效液相等多种色谱法对提取物进行分离纯化,并通过核磁共振和质谱技术鉴定化合物的结构。结果从柳叶蒿地上部分的70%乙醇提取物中分离鉴定了14个酚酸类成分,分别为苯甲醇-7-O-α-L-吡喃阿拉伯糖-(1→6)-β-D-吡喃葡萄糖苷(1)、金盏菊苷A(2)、对羟基苯甲酸(3)、原儿茶醛(4)、原儿茶酸(5)、3-甲氧基-4-羟基苯甲酸(6)、丁香酸(7)、丁香酸葡萄糖苷(8)、云杉苷(9)、scolochinenoside B(10)、2,6-羟基-4-甲氧基苯乙酮-2'-O-β-葡萄糖苷(11)、2-甲氧基-4-(2-烯丙基)苯基-β-D-吡喃葡萄糖苷(12)、4-烯丙基-2,6-二甲氧基苯基葡萄糖苷(13)和二氢丁香苷(14)。结论所有化合物均为首次从柳叶蒿中分离得到,其中化合物1、2、10、13和14为首次从蒿属植物中分离得到。

Objective To determine the chemical constituents from the aerial part of Artemisia integrifolia L..Methods Various chromatographic columns such as silica gel,ODS and HPLC were used to isolate and purify the constituents,whose structures were elucidated by NMR and MS spectra.Results As a result,14 phenolic derivatives were obtained,including benzyl primeveroside(1),calendnoside A(2),p-hydroxybenzoic acid(3),protocatechuic aldehyde(4),protocatechuic acid(5),3-methoxy-4-hydroxybenzoic acid(6),syringic acid(7),glucosyringic acid(8),picein(9),scolochinenoside B(10),domesticoside(11),2-methoxy-4-(2-propenyl)phenyl-β-D-glucopyranoside(12),4-allyl-2,6-dimethoxyphenol glucoside(13),and dihydrosyringin(14).Conclusion All of the compounds have been separated from Artemisia integrifolia L.for the first time.Among them,1,2,10,13 and 14 have been firstly obtained from Artemisia genus.