花生及其制品中黄曲霉毒素B1胶体金快速检测方法的建立

The Rapid Determination of Aflatoxin B1 in Peanut and Its Products by Colloidal Gold Method

通过考察市售试剂盒的绝对灵敏度和最大稀释比例,选取3种效果最好的快速检测胶体金试剂盒产品,以油炸花生、烘烤花生及花生酱为代表性基质,优化样品的前处理条件和试剂盒的反应条件,根据《食品快速检测方法评价技术规范》中性能指标进行统一的技术评价,考察各品牌快检试剂盒的检测限、特异性、灵敏度、假阳性率、假阴性率、相对准确度以及参比方法一致性分析等指标,为制定花生及其制品中黄曲霉毒素B1胶体金快速检测评价标准提供科学依据。

By investigating the absolute sensitivity and maximum dilution ratio of commercially available kits,three kinds of rapid detection kit products with the best effect were selected.Fried peanuts,roasted peanuts and peanut butter were taken as representative substrates to optimize the pre-treatment conditions of samples and the reaction conditions of kits.Unified technical evaluation was conducted according to the performance indexes in The Technical Specification for Evaluation of Rapid Food Detection Methods,and the detection limit,sensitivity,specificity,false negative rate,false positive rate,relative accuracy and consistency analysis of reference methods of all brands of rapid test kits were investigated.Our findings could provide scientific basis for the rapid determination and evaluation standard of aflatoxin B1 colloidal gold in peanut and its products.