深绿卷柏和江南卷柏的质量控制和抗氧化活性比较研究

Comparison on quality control and antioxidant activity between Selaginella doederleinii and S.moellendorffill

目的 对深绿卷柏和江南卷柏的质量控制和抗氧化活性进行比较。方法 对深绿卷柏和江南卷柏进行性状鉴别、显微鉴别和薄层色谱鉴别,采用HPLC法测定其中的穗花杉双黄酮,测定石油醚、醋酸乙酯和正丁醇部位的DPPH自由基清除率、ABTS^(+)·清除能力。结果 深绿卷柏和江南卷柏的性状、显微特征均有差异,薄层色谱中具有相同黄绿色荧光斑点,也有差异性;江南卷柏中穗花杉双黄酮的质量分数高于深绿卷柏;不同萃取部位的抗氧化活性的能力高低为醋酸乙酯>正丁醇>石油醚,且江南卷柏醋酸乙酯部位抗氧化活性优于深绿卷柏。结论 深绿卷柏和江南卷柏有显著差异,江南卷柏要比深绿卷柏抗氧化活性更好。

Objective To compare quality control and antioxidant activity between S.doederleinii and S.moellendorffii.Methods The character identification,microscopic identification,and TLC identification of S.doederleinii and S.moellendorffii were carried out.The contents of amentoflavone were determined by HPLC method,and the DPPH free radical scavenging rate and ABTS^(+)·scavenging capacity of petroleum ether,ethyl acetate and n-butanol fractions were measured.Results S.doederleinii and S.moellendorffii had differences in characters and microscopic characteristics,and TLC results of them had the same chartreuse fluorescent spots,but also have differences.The contents of amentoflavone in S.moellendorffii were higher than those in S.doederleinii.The antioxidant activities of different fractions were as follows:ethyl acetate>n-butanol>petroleum ether,and the antioxidant activity of the ethyl acetate fraction of S.moellendorffii was better than that of S.doederleinii.Conclusion There is a significant difference between S.doederleinii and S.moellendorffii,and S.moellendorffii has more antioxidant activities than S.doederleinii.