摘要
目的建立白芍中4个有效成分的含量测定方法,并采用主成分分析法评价不同产地白芍质量。方法使用乙醇超声提取白芍有效成分,使用WondaSiL WR C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-水,梯度洗脱,流速1 ml/min,柱温30℃,总运行时间65 min;将有效成分质量分数导入SPSS软件进行主成分分析,评价不同产地白芍质量。结果芍药苷、芍药内酯苷、苯甲酰氧化芍药苷和没食子酰芍药苷线性范围分别为0.0201~3.8200μg(r^(2)=0.9994)、0.0159~2.8500μg(r^(2)=0.9992)、0.0082~1.8200μg(r^(2)=0.9991)、0.0032~0.9700μg(r^(2)=0.9995)。10批白芍样品中,以产自安徽的白芍质量最佳,产自四川的白芍质量最差。结论建立了白芍HPLC测定方法并测定了4个有效成分含量,确定了安徽亳州为白芍主产区,可为白芍质量控制和制剂生产提供参考。
Objective To establish a method for the determination of four effective components in Paeoniae Radix Alba and evaluate its quality of Paeoniae Radix Alba by principal component analysis.Methods The effective components of Paeoniae Radix Alba were extracted by ultrasonic extraction method with ethanol.Wondasil WR C18 chromatographic column(250 mm×4.6 mm,5μm)was used,and the mobile phase was acetonitrile-water,the flow rate was set at 1 ml/min,the column temperature was set at 30℃,and the total operation time was 65 min.The mass score of active components was imported into SPSS for principal component analysis.Results The linear ranges of Paeoniflorin,Paeoniflorin,Benzoyl Paeoniflorin and Pallyl Paeoniflorin were 0.0201-3.8200μg(r^(2)=0.9994),0.0159-2.8500μg(r^(2)=0.9992),0.0082-1.8200μg(r^(2)=0.9991),0.0032-0.9700μg(r^(2)=0.9995).The quality of the 10 batches of Paeoniae Radix Alba samples from Anhui province was the best,while that from Sichuan province was the worst.Conclusions HPLC method was established for the determination of four effective components in Paeoniae Radix Alba,and principal component analysis and evaluation of 10 batches of Paeoniae Radix Alba.Bozhou,Anhui Province,was identified as the main production area of Paeoniae Radix Alba,which can provide reference for the quality control and preparation production of Paeoniae Radix Alba.
作者
蒋浩
王晓静
师超
Jiang Hao;Wang Xiaojing;Shi Chao(Department of Pharmacy,Medical Supplies Center of Chinese PLA General Hospital,Beijing 100048,China)
出处
《国际中医中药杂志》
2023年第5期600-603,共4页
International Journal of Traditional Chinese Medicine