摘要
盐分是降低作物产量的主要非生物胁迫因素.甜菜(Beta vulgaris)是耐盐性较强的经济作物,研究甜菜的耐盐机制具有重要意义.本研究以栽培甜菜O68品系为试验材料,利用m^(6)A-IP-qPCR探究了盐胁迫下m^(6)A修饰对甜菜耐盐基因CMO和NHX1表达的调控作用.在300 mM NaCl处理下,叶片中甘氨酸甜菜碱和Na+含量增加,编码CMO和NHX1的mRNA的表达水平升高、m^(6)A修饰水平降低、稳定性增强.这为m^(6)A修饰调控作物耐盐性提供了新的见解.
Salt is a major abiotic stress factor that reduces crop yield.Sugar beet(Beta vulgaris)is a cash crop with elevated salt tolerance,so it is crucial to study its salt tolerance mechanism.In this study,the cultivar beet 068 was used as experimental material,and the regulation effect of m^(6)A modification on the expression of salt-tolerant genes CMO and NHXI in beet under salt stress was investigated by m^(6)A-IP-qPCR.Upon treatment with 300 mM NaCl,the leaves showed increased glycine betaine and Na^(+)content,increased expression levels of CMO and NHXI,decreased m^(6)A modification levels of CMO and NHXI,and enhanced stability of CMO and NHXI mRNA.This study provided new insights into the modification of m^(6)A to modulate crop salt-tolerance.
作者
程晨曦
李俊良
李楠
CHENG Chen-xi;LI Jun-liang;LI Nan(National and Local Joint Engineering Research Center of Ecological Treatment Technology for Urban Water Pollution,Zhejiang Provincial Key Laboratory for Water Enwironment and Marine Biological Resources,College of Life and Environmental Science,Wenzhou University,Wenzhou,Zhejiang 325035)
出处
《中国甜菜糖业》
2023年第1期15-19,共5页
China Beet & Sugar
基金
温州市基础性科研项目重点实验室奖励项目(H20210004)。
