鳗鱼皮胶原蛋白水解物对结肠炎的改善作用

The Effect of Collagen Hydrolysate of Eel Skin on the Improvement of Colitis

溃疡性结肠炎(UC)是一种特发性肠道疾病,发病率不断上升,且治疗药物都存在不同程度的副作用。研究发现胶原蛋白肽可缓解UC症状,并且安全、健康。然而,目前研究主要局限于UC的预防作用,缺少对其治疗作用的研究。鳗鱼皮富含胶原蛋白,而鳗鱼头一般在加工中被丢弃,造成资源浪费,目前缺少对鳗鱼皮胶原蛋白水解物的研究。本研究尝试利用鳗鱼头中的鱼皮提取胶原蛋白并进行水解,希望得到对UC具有治疗作用的鳗鱼皮胶原蛋白水解物(CH),提高鳗鱼加工副产物的利用价值。研究发现鳗鱼皮所含胶原蛋白丰富,占鱼皮湿重的27.87%,纯度较高且主要为Ⅰ型胶原蛋白,可作为胶原蛋白的优质来源。对胶原蛋白进行酶解,得到具有抗氧化活性的CH,并给药UC模型小鼠,结果显示:与阳性对照和模型组相比,CH可以安全健康地降低UC模型小鼠的DAI评分至0,并改善结肠状态;结肠组织病理切片表明CH可以恢复黏膜上皮完整性,减轻炎性细胞浸润;结肠组织氧化应激指标表明CH可以降低结肠组织MDA含量(0.75 nmol/mg pro)和MPO活性(0.11 U/g tissue),并通过提高GSH含量(22.92 umol/g pro)和SOD(693.61 U/g pro)、CAT(1.69 U/g pro)、GPx(141.29U/g pro)3种抗氧化酶活性来提高结肠组织抗氧化能力,降低氧化应激水平,从而治疗UC。

Ulcerative colitis(UC)is an idiopathic intestinal disease with an increasing incidence,and all the therapeutic drugs have different degrees of side effects.Studies have found that collagen peptide can relieve the symptoms of UC,and is safe and healthy.However,the current research is mainly limited to the prevention effect of UC,and there is still a lack of research on the therapeutic effect of UC.Eel skin is rich in collagen,but eel head is generally discarded in processing,resulting in a waste of resources.Meanwhile,there is a lack of research on eel skin collogen hydrolysates.In this study,collagen was extracted from eel skin and hydrolyzed in the hope of obtaining eel skin collagen hydrolysate(CH)with therapeutic effect on UC,so as to improve the utilization value of eel processing by-products.It was found that eel skin contained abundant collagen,accounting for 27.87%of the wet weight of the skin.The extracted collagen was of high purity and mainly typeⅠcollagen,and eel skin could be used as a high-quality source of collagen.Collagen was enzymatically hydrolyzed to obtain eel skin collagen hydrolysate(CH)with antioxidant activity,and CH was administered to DSS induced UC mouse model.The results showed that CH could safely and healthily reduce DAI score to 0 and improve colon status in UC Model mice compared with Positive control group and Model group.Pathological sections of colon tissue showed that CH could restore the integrity of mucosal epithelium and reduce inflammatory cell infiltration.Oxidative stress index of colon tissue showed that CH could reduce MDA content(0.75 nmol/mg protein)and MPO activity(0.11 U/g tissue)in colon tissue,and improve the antioxidant capacity of colon tissue and reduce oxidative stress level by increasing GSH content(22.92 umol/g pro)and activities of SOD(693.61 U/g pro),CAT(1.69 U/g pro)and GPx(141.29 U/g pro)antioxidant enzymes,so as to treat UC.