白玉菇多糖发酵乳对小鼠肠道微生物的影响及抗氧化作用

Effects of Polysaccharide Fermented Milk from White Hypsizygusmarmoreus on Intestinal Microflora in Mice and Antioxidant Capacity

在发酵乳中添加白玉菇水提多糖(WHN)和酶提多糖(WHE),研究白玉菇多糖发酵乳对小鼠肠道的保护作用,对肠道菌群的调节和抗氧化作用。结果表明:白玉菇多糖发酵乳增加了小鼠粪便含水量,白玉菇酶提多糖高剂量组小鼠结肠指数为1.62,结肠长度为9.59 cm,相比于空白组显著增加,白玉菇酶提多糖高剂量组结肠内容物中短链脂肪酸含量为26.45mmol/L,是空白组的1.38倍,其中还原糖和单糖的含量显著增加。Alpha多样性指数分析表明,白玉菇水提多糖高剂量发酵乳组小鼠肠道菌群物种丰度最高,从门水平和属水平分析,增加了拟杆菌门、放线菌门以及乳酸菌属丰度。白玉菇酶提多糖高剂量组小鼠的抗高血脂和抗氧化效果最好,提高了小鼠T-AOC、SOD、CAT活性,同时降低了MDA含量。白玉菇多糖发酵乳通过改善小鼠肠道生理状况来调节肠道菌群结构,增强抗氧化能力,其中WHE发酵乳组效果优于WHN发酵乳组,有一定的剂量依赖性。

To explore the protective effects of polysaccharide fermented milk from White Hypsizygusmarmoreus on intestinal tract,intestinal flora and antioxidant of mice,the polysaccharide fermented milk was prepared by using water extracted polysaccharide(WHN)and enzyme extracted polysaccharide(WHE)as experimental materials.The results showed that polysaccharide fermented milk increased fecal water content of mice.Colon index and colon length of mice in high-dose polysaccharide extraction group were 1.62 and 9.59 cm,which were significantly increased compared with blank group.Short chain fatty acids content in colon contents of high-dose polysaccharide extraction group was 26.45 mmol/L,1.38 times of that in blank group.The contents of reducing sugars and monosaccharides increased significantly.Moreover,the intestinal flora species abundance in the group of the high dosage of fermented milk with WHN was highest,and the abundances of Bacteroidetes,Actinobacteria and Lactobacillus were increased at the genus and phylum levels by analyzing the Alpha diversity indexes.While the effects of the high dosage of fermented milk with WHE on antioxidant and anti-hyperlipidemic were the best,it could increase the activities of T-AOC,SOD and CAT,decrease the content of MDA.The findings indicated that the polysaccharide fermented milk from White Hypsizygusmarmoreus could play a key role in improving the intestinal physiology,regulating gut microbiota and enhancing antioxidant capacity in mice,providing research ideas and a theoretical basis for the polysaccharide fermented milk of from White Hypsizygusmarmoreus,WHE fermented milk group is better than WHN fermented milk group,in a certain dose dependence.