3种新疆沙棘黄酮的提取优化及抗氧化活性对比

Optimization of Extraction of Flavonoids from Three Kinds of Xinjiang Sea Buckthorns and Comparative of Antioxidant Activities

采用超声波辅助法提取沙棘果实中的总黄酮,采用单因素实验和响应面试验优化提取工艺,采用优化的工艺提取不同成熟度、不同地区沙棘果实黄酮,采用高效液相色谱法分析4种主要黄酮苷元的分布及含量,同时比较这些沙棘黄酮提取物的抗氧化活性。结果表明:乙醇体积分数55%、料液比1∶45(g/mL)、超声功率210 W时,沙棘A的总黄酮提取率最高可达3.241%。沙棘果实中主要的黄酮苷元为异鼠李素、槲皮素、山奈酚、杨梅素,而3种沙棘果实黄酮苷元的含量有差异。抗氧化活性测定结果表明,沙棘C的黄酮提取物具有更强的1,1-二苯基-2-三硝基苯肼自由基清除率、2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基清除率和还原能力,总黄酮苷元含量与DPPH自由基清除率、还原能力呈显著正相关。本研究结果为沙棘黄酮的综合利用开发提供数据支持。

In this study,ultrasonic-assisted method was used to extract total flavonoids from sea buckthorn fruits.The extraction process was optimized by the single factor test and the response surface test.The distribution and contents of four main flavonoid aglycones from sea buckthorn fruits of different maturities and different regions were determined by the optimized extraction process combined with high-performance liquid chromatography.Thereafter a comparative study of the antioxidant activity of sea buckthorn flavonoids extracts was carried out.The optimal extraction conditions were determined as follows:ethanol concentration 55%,solid-to-solvent ratio 1∶45(g/mL),and ultrasonic power 210 W.The extraction rate of sea buckthorn A total flavonoids was up to 3.241%under the optimized conditions.The main flavonoid aglycones in sea buckthorn fruits were isorhamnetin,quercetin,kaempferol and myricetin,and the content of flavonoid aglycones varied with the maturity and region of the fruit.Total flavonoids extract from the sea buckthorn C fruits exhibited strong antioxidant activities as determined by 1,1-diphenyl-2-picrylhydrazyl(DPPH),2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)radical scavenging and ferric reducing antioxidant power assays.The total flavonoid aglycones content was significantly positively correlated with the DPPH radical scavenging capacity and ferric reducing power.In summary,this study can provide a scientific basis for the comprehensive utilization and development of sea buckthorn fruits.