ING4、Fas和FasL在膀胱尿路上皮癌中的表达及临床病理意义

Expression and clinical pathology significance of ING4,Fas and FasL in bladder urothelial carcinoma

目的探讨膀胱尿路上皮癌(BUC)组织中生长抑制因子4(ING4)、Fas和FasL的表达变化及意义。方法收集2017年至2020年经膀胱镜活检或手术切除病理诊断为BUC的60例BUC组织(BUC组),同时收集该组患者的癌旁组织(癌旁组)。采用免疫组化法分别检测BUC组织和癌旁组织中ING4、Fas和FasL的表达,分析其与病理分级、临床分期及临床资料的关系。采用Spearman等级相关分析BUC组织中ING4、Fas和FasL的相关性。结果BUC组的ING4阳性表达率低于癌旁组[70%(42/60)vs.100%(60/60),P<0.05]。BUC中的Fas阳性表达率低于癌旁组[70%(42/60)vs.88.3%(53/60),P<0.05]。BUC组的FasL阳性表达率低于癌旁组[76.7%(46/60)vs.100%(60/60),P<0.05]。随着BUC病理分级的分化程度或临床分期的增高,ING4在BUC组织中的表达呈下降趋势(P<0.05)。随着BUC病理分级增高,Fas在BUC组织中的表达呈下降趋势(P<0.05)。BUC中的FasL表达与患者的临床分期、病理分级无相关性(均P>0.05)。ING4与Fas、FasL呈正相关,差异均有统计学意义(均P<0.05)。结论ING4、Fas和FasL在BUC与癌旁组织中的阳性表达存在显著差异,提示ING4可能在促进Fas凋亡系统中起重要作用。介导ING4和Fas凋亡的关系可能成为BUC新的免疫治疗靶点。

Objective To investigate the expression and clinical pathology significance of inhibitor of growth family member 4(ING4),Fas and FasL in bladder urothelial carcinoma(BUC).Methods From 2017 to 2020,60 patients diagnosed with BUC by cystoscopy biopsy or surgical resection(BUC group),at the same time,the paracancer tissues of the patients(paracancer group)were collected.The expressions of ING4,Fas and FasL in BUC and paracancer tissues were detected by immunohistochemical method,and their relationship with pathological grade,clinical stage and clinical data were analyzed.Spearman rank correlation was used to analyze the correlation of ING4,Fas and FasL in BUC.Results The positive expression rate of ING4 in BUC was lower than that in paracancer tissues[70%(42/60)vs.100%(60/60),P<0.05].The positive expression rate of Fas in BUC was lower than that in adjacent tissues[70%(42/60)vs.88.3%(53/60),P<0.05].The positive expression rate of FasL in BUC was lower than that in paracancer tissues[76.7%(46/60)vs.100%(60/60),P<0.05].The expression of ING4 in BUC decreased with the increase of the differentiation degree of pathological grade or clinical stage of BUC(P<0.05).The expression of Fas in BUC decreased with the increase of the pathological grade(P<0.05).The expression of FasL in BUC was not correlated with the clinical stage and pathological grade of the patients(all P>0.05).ING4 was positively correlated with Fas and FasL,and the differences were statistically significant(all P<0.05).Conclusions The positive expressions of ING4,Fas and FasL in BUC were significantly different from those in paracancer tissues,suggesting that ING4 may play an important role in promoting Fas apoptosis.The relationship between ING4 and Fas apoptosis may be a new immunotherapeutic target for BUC.