采用常压室温等离子体诱变技术选育高产多糖黑木耳菌株

Breeding of Auricularia auricula Strains with High Polysaccharide Yield by ARTP Mutagenesis

为选育高产多糖的黑木耳(Auricularia auricula)菌株,以黑威单片作为出发菌株,采用常压室温等离子体(atmospheric and room temperature plasma,ARTP)诱变原生质体,经过3轮迭代诱变,从菌丝生长速度较快的诱变菌株中筛选多糖产量较高的菌株,通过5代继代培养测定菌株的菌丝生长速度稳定性,采用拮抗实验观察菌株间的拮抗情况,通过简单重复序列区间(inter-simple sequence repeat,ISSR)分子标记分析菌株间的遗传差异。结果表明:与黑威单片相比,诱变菌株D3-56的菌丝生物量和多糖含量分别提高15.02%和48.40%,诱变菌株D3-60的菌丝生物量和多糖含量分别提高13.15%和60.83%,诱变菌株D3-56和D3-60的多糖产量分别提高70.69%和81.97%;诱变菌株D3-56和D3-60菌丝生长速度稳定,且与黑威单片存在遗传差异。

In order to screen superior Auricularia auricula strains with high polysaccharide yield,A.auricula Heiwei danpian was subjected to atmospheric and room temperature plasma(ARTP)mutagenesis for three rounds.Then mutant strains with high polysaccharide yield were selected out of mutants showing fast mycelial growth.The selected mutant strains were then subcultured for five passages rate to determine the stability of mycelial growth rate.Antagonism between the selected strains were observed by antagonistic experiment,and the genetic variances of the selected strains were analyzed by ISSR markers.The results showed that compared with the starting strain Heiwei danpian,the biomass and polysaccharide content of the mutant strain D 3-56increased by 15.02%and 48.40%,respectively;the biomass and polysaccharide content of the mutant strain D3-60 increased by 13.15%and 60.83%,respectively;and the polysaccharide yields of D3-56 and D3-60 increased by 70.69%and 81.97%,respectively.After five subcultures,D3-56 and D3-60 showed stable mycelial growth and genetic differences with Hewei danpian.