桂林地区危重患者弓形虫感染情况调查与DNA鉴定分析

Investigation and DNA identification for Toxoplasma gondii infection in critical patients in Guilin region

目的:了解广西桂林地区危重患者弓形虫感染情况。方法:选取2018年11月至2019年11月在桂林医学院附属医院住院的危重患者260例,采集患者空腹静脉血,分离血清,采用ELISA法检测血清弓形虫IgG抗体;分析弓形虫感染与性别、年龄和患病类型间的相关性;采用PCR技术对弓形虫感染阳性患者进行DNA鉴定。结果:260例危重患者,弓形虫感染IgG阳性男73例、女24例,弓形虫IgG阳性检出率为37.31%;男患者弓形虫IgG阳性率41.01%,高于女患者29.27%;弓形虫感染与危重患者性别、年龄和患病类型无相关性(P>0.05);弓形虫IgG阳性患者血液标本中没有检测到弓形虫特异性DNA序列。结论:桂林地区危重患者弓形虫感染率较高,年龄、患病类型不是影响弓形虫感染的危险因素。

Objective:To investigate the infection status of T.gondii in critical patients in the area of Guilin,Guangxi.Methods:260 fasting venous blood samples of critical patients admitted in the Affiliated Hospital of Guilin Medical University from Nov.2018 to Nov.2019 were collected,then the serum was separated,and the serum IgG antibody level of T.gondii was detected by ELISA method.The correlation between T.gondii infection and gender,age and type of disease was analyzed,and all T.gondii IgG positive samples were identified through DNA extraction.Results:Our data showed that there were 73 male and 24 female critical patients with T.gondii IgG antibodies can be positive infection,with the seroprevalence rate of 37.31%;the seroprevalence rate in male was 41.01%,which is higher than 29.27%in female;and there was no correlation between T.gondii infection and gender,age and disease type of critical patients(P>0.05)and no T.gondii-specific DNA sequences were detected in the serum samples of patients with positive IgG.Conclusion:The infection rate of T.gondii is higher in critical patients in Guilin region,but the age and type of disease are not the risk factors for the infection.