数字PCR技术在体液中检测恶性肿瘤的研究进展

Research progress of digital PCR technology in the detection of malignant tumors in body fluids

恶性肿瘤的诊断与治疗已经日渐趋向个体化医疗阶段,要求临床工作者对恶性肿瘤多变的生长进化过程和复杂的内部分子结构以及耐药的产生要有综合全面的把握。基于体液检测的数字PCR技术,因为其取材方便,可有效规避组织活检带来的有创损伤以及较高的检测灵敏度和特异性,所以能够在体液样本中检测到恶性肿瘤的相关基因,有望成为恶性肿瘤分子检测的可靠工具之一。本文就数字PCR技术在体液中检测恶性肿瘤的研究进行综述。

The diagnosis and treatment of malignant tumors have been moving towards the stage of individualized medicine,which requires clinical workers to have a comprehensive grasp of the variable growth and evolution process of malignant tumors,the complex internal molecular structure and the generation of drug resistance.Digital PCR technology based on body fluids detection is expected to become one of the reliable tools for molecular detection of malignant tumors because of its convenient sampling,effective avoidance of invasive damage caused by tissue biopsy and high detection sensitivity and specificity.Therefore,it can detect malignant tumor related genes in body fluid samples.This article mainly reviews the researches on the detection of malignant tumors in body fluids by digital PCR technology.