响应面法优化苍术低聚果糖提取工艺

Optimizing the extraction of fructooligosaccharides from Atractylodes lancea by response surface methodology

采用超声辅助提取,响应面法优化苍术中低聚果糖(FOS)的提取工艺。在单因素实验的基础上,以提取时间、超声温度和提取料液比为优化因素,FOS总提取率为评价指标,采用Box-Behnken中心模型设计三因素三水平进行响应面优化,高效液相蒸发光检测法检测。结果表明苍术FOS最优提取工艺为超声温度70℃、提取时间60 min、料液比为1∶40,在此条件下FOS总提取率为2.44%,蔗果三糖、蔗果四糖、蔗果五糖、蔗果六糖、蔗果七糖、蔗果八糖和蔗果十糖提取率分别为0.94%、0.33%、0.21%、0.23%、0.43%、0.17%、0.12%。

Ultrasound-assisted extraction and response surface methodology were used to optimize the extraction process of fructooligosaccharides(FOS)in Atractylodes rhizoma.On the basis of single factor experiment,extraction time,extraction temperature and extraction solid-liquid ratio were selected as influence factors,and total yield of FOS as the evaluation index.Box-Behnken central model was used to design three factors and three levels response surface optimization experiment.High performance liquid evaporative light detection method was used.The optimal extraction process was as folllows:extraction temperature 70℃,extraction time 60 min,and solid-liquid ratio 1∶40.Under these conditions,the total yield of FOS was 2.44%;the yields of GF2,GF3,GF5,GF5,GF6,GF7,GF8,and GF9 were 0.94%,0.33%,0.21%,0.23%,0.43%,0.17%,and 0.12%,respectively.